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The most beautiful frog in Japan and conservation using an artificial breeding technique

Ishikawa’s frog (Rana ishikawae) is considered to be the most beautiful in Japan, and is designated as a protected species in Kagoshima and Okinawa Prefectures, and also as an endangered species in the Red List of the Ministry of the Environment. We are now attempting to conserve this species by artificial breeding in the laboratory (Fig. 15, Fig. 16, Fig. 17).


Fig. 15. Ovulated eggs.



Fig. 16. Artificial insemination.



Fig. 17. Three-year-old Ishikawa’s frogs produced by artificial breeding.

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