ー植物の環境適応と成長生存戦略ー 分子形質発現学研究室HPへようこそ

研究業績

原著論文

• Watanabe, S., Sato, M., Sawada, Y., Tanaka, M., Matsui, A., Kanno, Y., Hirai, M., Seki, M., Sakamoto, A., Seo, M. (2018) Arabidopsis molybdenum cofactor sulfurase ABA3 contributes to anthocyanin accumulation and oxidative stress tolerance in ABA-dependent and independent ways. Sci. Rep. 8:16592. OPEN ACCESS 　

• Takagi, H., Watanabe, S., Tanaka, S., Matsuura, T., Mori, I.C., Hirayama, T., Shimada, H., Sakamoto, A. (2018) Disruption of ureide degradation affects plant growth and development during and after transition from vegetative to reproductive stages. BMC Plant Biol. 18:287. OPEN ACCESS

• Tominaga, J., Nakahara, Y., Horikawa, D., Tanaka, A., Kondo, M., Kamei, Y., Takami, T., Sakamoto, W., Unno, K., Sakamoto, A., Shimada, H. (2018) Overexpression of the protein disulfide isomerase AtCYO1 in chloroplasts slows dark-induced senescence in Arabidopsis. BMC Plant Biol. 18:10. OPEN ACCESS

• Takahashi, M., Shigeto, J., Sakamoto, A., Morikawa, H. (2017) Selective nitration of PsbO1, PsbO2, and PsbP1 decreases PSII oxygen evolution and photochemical efficiency in intact leaves of Arabidopsis. Plant Signal. Behav. 12:e1376157.

• Takahashi, M., Shigeto, J., Sakamoto, A., Morikawa, H. (2017) Selective nitration of PsbO1 inhibits oxygen evolution from isolated Arabidopsis thylakoid membranes. Plant Signal. Behav. 12:e1304342.

• Takahashi, M., Shigeto, J., Sakamoto, A., Morikawa, H. (2016) Light-triggered selective nitration of PSBO1 in isolated Arabidopsis thylakoid membranes is inhibited by photosynthetic electron transport inhibitors. Plant Signal. Behav. 11:e1263413.

• Tominaga, J., Mizutani, H., Horikawa, D., Nakahara, Y., Takami, T., Sakamoto, W., Sakamoto, A, Shimada, H. (2016) Rice CYO1, an ortholog of Arabidopsis thaliana cotyledon chloroplast biogenesis factor AtCYO1, is expressed in leaves and involved in photosynthetic performance. J. Plant Physiol. 207:78-83.

• Takahashi, M., Shigeto, J., Shibata, T., Sakamoto, A., Morikawa, H. (2016) Differential abilities of nitrogen dioxide and nitrite to nitrate proteins in thylakoid membranes isolated from Arabidopsis leaves. Plant Signal. Behav. 11:e1237329.

• Takagi, H., Ishiga, Y., Watanabe, S., Konishi, T., Esuga, M., Akiyoshi, N., Matsuura, T., Mori, I.C., Hirayama, T., Kaminaka, H., Shimada, H., Sakamoto, A. (2016) Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner. J. Exp. Bot. 67:2519-2532. OPEN ACCESS

• Takahashi, M., Shigeto, J., Sakamoto, A., Izumi, S., Asada, K., Morikawa, H. (2015) Dual selective nitration in Arabidopsis: Almost exclusive nitration of PsbO and PsbP, and highly susceptible nitration of four non‐PSII proteins, including peroxiredoxin II E. Electrophoresis 36:2569-2578.

• Sakamoto, A., Nishimura, T., Miyaki, Y., Watanabe, S., Takagi, H., Izumi, S., Shimada, H. (2015) In vitro and in vivo evidence for oxalate oxidase activity of a germin-like protein from azalea. Biochem. Biophys. Res. Commun. 458:536-542.

• Watanabe, S., Kounosu, Y., Shimada, H., Sakamoto, A. (2014) Arabidopsis xanthine dehydrogenase mutants defective in purine degradation show a compromised protective response to drought and oxidative stress. Plant Biotechnol. 31:173-178.

• Watanabe, S., Matsumoto, M., Hakomori, Y., Takagi, H., Shimada, H., Sakamoto, A. (2014) The purine metabolite allantoin enhances abiotic stress tolerance through synergistic activation of abscisic acid metabolism. Plant Cell Environ. 37:1022-1036.

• Takahashi, M., Furuhashi, T., Ishikawa, N., Horiguchi, G., Sakamoto, A., Tsukaya, H., Morikawa, H. (2014) Nitrogen dioxide regulates organ growth by controlling cell proliferation and enlargement in Arabidopsis. New Phytol. 201:1304-1315.

• Nakano, S., Takahashi, M., Sakamoto, A., Morikawa, H., Katayanagi, K. (2012) X-ray crystal structure of a mutant assimilatory nitrite reductase that shows sulfite reductase activity-like activity. Chem. Biodivers. 9:1989-1999.

• Muranaka, A., Watanabe, S., Sakamoto, A., Shimada, H. (2012) Arabidopsis cotyledon chloroplast biogenesis factor CYO1 uses glutathione as an electron donor and interacts with PSI (A1 and A2) and PSII (CP43 and CP47) subunits. J. Plant Physiol. 169:1212-1215.

• Nakano, S., Takahashi, M., Sakamoto, A., Morikawa, H., Katayanagi, K. (2012) The reductive reaction mechanism of tobacco nitrite reductase derived from a combination of crystal structures and UV-Vis microspectroscopy. Proteins 80:2035-2045.

• Morita, S., Tsukamoto, S., Sakamoto, A., Makino, H., Nakauji, E., Kaminaka, H., Masumura, T., Ogihara, Y., Satoh, S., Tanaka, K. (2012) Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants. Plant Biotechnol. 29:115-119.

• Nakano, S., Takahashi, M., Sakamoto, A., Morikawa, H., Katayanagi, K. (2012) Structure-function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on the high resolution structure. Protein Sci. 21:383-395.

• Takahashi, M., Sakamoto, A., Ezura, H., Morikawa, H. (2011) Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants. Plant Biotechnol. 28:485-487.

• Watanabe, S., Nakagawa, A., Izumi, S., Shimada, H., Sakamoto, A. (2010) RNA interference-mediated suppression of xanthine dehydrogenase reveals the role of purine metabolism in drought tolerance in Arabidopsis. FEBS Lett. 584:1181-1186.

• Shimada, H., Obayashi, T., Takahashi, N., Matsui, M., Sakamoto, A. (2010) Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells. Plant Methods 6:29.

• Kondo, K., Yamada, K., Nakagawa, A., Takahashi, M., Morikawa, H., Sakamoto, A. (2008) Molecular characterization of atmospheric NO2-responsive germin-like proteins in azalea leaves. Biochem. Biophys. Res. Commun. 377:857-861.

• Adam, S.E.H., Shigeto, J., Sakamoto, A., Takahashi, M., Morikawa, H. (2008) Atmospheric nitrogen dioxide at ambient levels stimulates growth and development of horticultural plants. Botany 86:216-217.

• Abdel-Banat, B.M.A., Adam, S.E.H., Takahashi, M., Sakamoto, A., Shoun, H., Morikawa, H. (2008) A fungal cytochrome P-450nor confers denitrifying ability to tobacco BY-2 cells. Biotechnology 7:250-257.

• Adam, S.E.H., Abdel-Banat, B.M.A., Sakamoto, A., Takahashi, M., Morikawa, H. (2008) Effect of atmospheric nitrogen dioxide on mulukhiya (Corchorus olitorius) growth and flowering. Am. J. Plant Physiol. 3:180-184.

• Nakagawa, A., Sakamoto, S., Takahashi, M., Morikawa, H., Sakamoto, A. (2007) The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic Arabidopsis plants. Plant Cell Physiol. 48:1484-1495.

• Shigeto, J., Yoshihara, S., Adam, S.E.H., Sueyoshi, K., Sakamoto, A., Morikawa, H., Takahashi, M. (2006) Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino. Plant Biotechnol. 27:99-105.

• Kurumata, K., Takahashi, M., Sakamoto, A., Ramos, J.L., Nepovim, A., Vanek, T., Hirata, T., Morikawa, H. (2005) Tolerance to, and uptake and degradation of 2,4,6-trinitrotoluene (TNT) are enhanced by the expression of a bacterial nitroreductase gene in Arabidopsis thaliana. Z. Naturforsch. [C] 60:272-278.

• Morikawa, H., Takahashi, M., Sakamoto, A., Ueda-Hashimoto, M., Matsubara, T., Miyawaki, K., Kawamura, Y., Hirata, T., Suzuki, H. (2005) Novel metabolism of nitrogen in plants. Z. Naturforsch. [C] 60:265-271.

• Takahashi, M., Tsukamoto, S., Kawaguchi, A., Sakamoto, A., Morikawa, H. (2005) Phytoremediators from abandoned rice field. Plant Biotechnol. 22:167-170.

• Hokazono, H., Takahashi, M., Sakamoto, A., Morikawa, H. (2005) TJ1 is an orientation-independent transformation enhancer sequence. Plant Biotechnol. 22:137-140.

• Takahashi, M., Konaka, M., Sakamoto, A., Morikawa, H. (2005) Nocturnal uptake and assimilation of nitrogen dioxide by C3 and CAM plants. Z. Naturforsch. [C] 60:279-284.

• Takahashi, M., Nakagawa, M., Sakamoto, A., Ohsumi, C., Matsubara, T., Morikawa, H. (2005) Atmospheric nitrogen dioxide gas is a plant vitalization signal to increase plant size and the contents of cell constituents. New Phytol. 168:149-15.

• Sakamoto, A., Sakurao, S.-H., Fukunaga, K., Matsubara, T., Ueda-Hashimoto, M., Tsukamoto, S., Takahashi, M., Morikawa, H. (2004) Three distinct Arabidopsis hemoglobins exhibit peroxidase-like activity and differentially mediate nitrite-dependent protein nitration. FEBS Lett. 572:27-32.

• Sakamoto, A., Sulpice, R., Hou, C.-X., Kinoshita, M., Higashi, S.-I., Kanaseki, T., Nonaka, H., Moon, B.Y., Murata N. (2004) Genetic modification of fatty acid unsaturation of phosphatidylglycerol in chloroplasts alters the sensitivity of tobacco plants to cold stress. Plant Cell Environ. 27:99-105.

• Park, E.-J., Jeknic, Z., Sakamoto, A., DeNoma, J., Yuwansiri, R., Murata, N., Chen, T.H.H. (2004) Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants and flowers from chilling damage. Plant J. 40:474-487.

• Morikawa, H., Takahashi, M., Sakamoto, A., Matsubara, T., Arimura, G.-I., Kawamura, Y., Fukunaga, K., Fujita, K., Sakurai, N., Hirata, T., Ide, H., Nonoyama, N., Suzuki, H. (2004) Formation of unidentified nitrogen in plants: an implication for a novel nitrogen metabolism. Planta 219:14-22.

• Kato, C., Takahashi, M., Sakamoto, A., Morikawa, H. (2004) Differential expression of the nitrite reductase gene family in tobacco as revealed by quantitative competitive RT-PCR. J. Exp. Bot. 55:1761-1763.

• Sakamoto, A., Tsukamoto, S., Yamamoto, H., Ueda-Hashimoto, M., Takahashi, M., Suzuki, H., Morikawa, H. Functional complementation in yeast reveals a protective role of chloroplast 2-Cys peroxiredoxin against reactive nitrogen species. Plant J. 33:841-851.

• Hakata, M., Takahashi, M., Zumft, W., Sakamoto, A., Morikawa, H. (2003) Conversion of the nitrate nitrogen and nitrogen dioxide to nitrous oxides in plants. Acta Biotechnol. 23:249-257.

• Erkin, O.C., Takahashi, M., Sakamoto, A., Morikawa, H. (2003) Development of regeneration and transformation systems for Raphiolepis umbellata L. plants using particle bombardment. Plant Biotechnol. 20:145-152.

• Sakamoto, A., Ueda, M., Morikawa, H. (2002) Arabidopsis glutathione-dependent formaldehyde dehydrogenase is an S-nitrosoglutathione reductase. FEBS Lett. 515:20-24.

• Mohanty, A., Kathuria, H., Ferjani, A., Sakamoto, A., Mohanty, P., Murata, N., Tyagi, A.K. (2002) Transgenics of an elite indica rice variety Pusa Basmati 1 harbouring codA gene are highly tolerant to salt stress. Theor. Appl. Genet. 106:51-57.

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総説・著書

• Takahashi, M., Matsubara, T., Sakamoto, A., Morikawa. H. (2007) Uptake, assimilation and novel metabolism of nitrogen dioxide in plants. In: Methods in Biotechnology Vol. 23: Phytoremediation Methods and Review (N.J. Willey, ed.), pp. 109-118. Humana Press, Totowa, NJ.

• Morikawa, H., Takahashi, M., Hakata, M., Matsubara, T., Sakamoto, A. (2006) Higher plants and metabolism of oxides of nitrogen. In: Focus on Plant Molecular Biology 2: Biotechnological Approaches to Improve Nitrogen Use Efficiency in Plants (R.P. Sigh, P.K. Jaiwal, eds.), pp. 103-133. Studium Press LLC, Houston, TX.

• Park, E.-J., Jeknic, A., Sakamoto, A., DeNoma, J., Murata, N., Chen, T.H.H. (2003) Genetic engineering of cold-tolerant tomato via glycinebetaine biosynthesis. Cryobiol. Cryotechnol. 49:77-85.

• Morikawa, H., Takahashi, M., Hakata, M., Sakamoto, A. (2003) Screening and genetic manipulation of plants for decontamination of pollutants from the environments. Biotechnol. Adv. 22:9-15.

• Sakamoto, A., Murata, N. (2002) The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant Cell Environ. 25:163-171.

• Morikawa, H., Sakamoto, A., Hokazono, H., Irifune, K., Takahashi, M. (2002) Mechanism of transgene integration into a host genome by particle bombardment. Plant Biotechnol. 19:219-228.

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和文総説・著書

• 渡邊俊介，坂本　敦 (2018) 植物のプリン分解 ― 最近の進展と見えてきたストレス適応における役割．植物の生長調節 53:116-123.

• 中村典子，坂本 敦，小野 道之，水谷正子，田中良和 (2014) 遺伝子組換えカーネーション「ムーンダスト」を用いた導入遺伝子の検出と導入遺伝子に由来するアントシアニジンの検出のためのプロトコール（オンライン版．日本植物細胞分子生物学会)

• 坂本　敦，松原俊之，高橋美佐，森川弘道 (2004) 活性窒素代謝 －植物窒素代謝の新しい課題－．日本農芸化学会誌 78:958-981.

• 坂本　敦 (2003) 光合成事典 (日本光合成研究会編)，学会出版センター，東京 (分担執筆)．

• 森川弘道，坂本　敦，高橋美佐 (2003) 窒素同化とNOx代謝 ．蛋白質 核酸 酵素・増刊「植物の代謝コミュニケーション：植物分子生理学の新展開」(編集：杉山達夫，水野　猛，長谷俊二，斉藤和季)，48:2130-2137.

• 坂本　敦，高橋美佐，森川弘道 (2002) 植物細胞の膜輸送系とファイトレメディエーション．細胞工学別冊・植物細胞工学シリーズ18「植物の膜輸送システム．ポンプ・トランスポーター・チャネル研究の新展開」 (監修：加藤 潔，島崎研一郎，前島正義，三村徹郎)，pp. 146-150，秀潤社，東京.

• 坂本　敦，村田紀夫 (2002) 環境耐性能の改変．シリーズ・バイオサイエンスの新世紀・第15巻「生命工学 － 新しい生命へのアプローチ」 (日本生化学会編)，pp. 156-164，共立出版，東京.

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