English is the official language of the symposium.
No interpretation services will be provided.
The following sessions are planned for oral and poster presentations:
2. Epidemiology and Pathogenesis
3. Virology and Molecular Biology
4. Disease Control
5. Miscellaneous Topics
November 28, 2006 (Tue)
Registration and Welcome Reception at Mitsui
Garden Hotel Hiroshima
(CONFRERE; 25 F)
November 29, 2006 (Wed)
Special Lecture by Dr. Kyle L. Johnson (The University of Texas at El Paso)
Poster Sessions (during Lunch Break)
Round Table Discussion (after Oral Sessions)
November 30, 2006 (Thu)
Oral Sessions (during Lunch Break)
Business Meeting (during Lunch Break)
Symposium Banquet at ANA Hotel Hiroshima
December 1, 2006 (Fri)
Sympsoium Tour : A-Bomb Dome, National Research Institute of Fisheries
and Environment of Inland Sea, and Miyajima where a Wolrd Cultural Heritage,
"Itsukushima Shinto Shrine" stands.
Kyle L. Johnson, Ph.D.
(The University of Texas at El Paso)
Alphanodavirus RNA replication and suppression
of RNA interference by protein
Dr. Kyle L. Johnson obtained a Bachelor of
Science in Biology from the University of Washington (Seattle) in 1985. At that time, she became interested
in virology and worked with Drs. Akiko Hirano
and Tim Wong at the University of Washington and with Dr. Maxine Linial at the Fred Hutchinson Cancer Research Center (Seattle, WA). Her research explored the mechanisms by
which avian retroviruses became integrated
into the host genome. In 1987, she entered
the Ph.D. program in Molecular Biology at
the University of Colorado Health Sciences
Center in Denver, CO. Under the supervision of Dr. Peter Sarnow
(now at Stanford University), her dissertation research focused on the
replication of poliovirus RNA.
In 1994, she began postdoctoral training
in the Department of Biochemistry at the
University of Alabama at Birmingham (UAB). In the laboratory of
Drs. Thomas R. Broker and Louise T. Chow,
she performed site-directed mutagenesis of
several coding regions within the DNA genome
of human papillomavirus (HPV) type 11, as an initial step toward
establishing a reverse genetic system for
HPV. Dr. Johnson returned to RNA virology
in 1995 when she moved to the UAB Department
of Microbiology for a second postdoctoral
fellowship in the laboratory of Dr. L. Andrew
Ball. There, her research focused on RNA
replication of the alphanodavirus Flock House virus in transfected mammalian cells.
In 1997, Dr. Johnson accepted a position
as a Research Assistant Professor in the
Department of Microbiology at UAB. While
maintaining her collaboration with Dr. Ball’s
laboratory, Dr. Johnson shifted her research
focus to Nodamura virus (NoV), the type species of the alphanodaviruses.
She established a reverse genetic system
for NoV, using cloned cDNA copies of NoV
genomic RNAs, which functions in a number
of cultured mammalian and insect cell lines
and in cells of the yeast Saccharomyces cerevisiae. These reagents have facilitated the study
of NoV RNA replication and allowed characterization
of NoV mutants, including those unable to
synthesize the nonstructural protein B2.
B2-deficient mutants showed defects in RNA
accumulation in both mammalian and insect
cells. The severity of these defects differed
in a cell-specific manner that correlated
with the cell’s ability to mount an RNA
interference (RNAi) response. Her collaboration
with Dr. Shou-Wei Ding and others showed
that NoV B2 suppresses RNAi in transfected
insect cells. While at UAB, Dr. Johnson also
served as an Associate Scientist in the UAB
Comprehensive Cancer Center (1999-2004),
an Associate Member of UAB’s Center for
AIDS Research (CFAR; 2001-2004), and an Associate
Scientist in UAB’s Gregory Fleming James
Cystic Fibrosis Research Center (2002-2004).
In October 2004, Dr. Johnson joined the faculty
of the University of Texas at El Paso (UTEP) as an Assistant Professor
in the Department of Biological Sciences.
Her research continues to focus on the mechanism
of nodavirus RNA replication, the role of
the B2 protein in the nodavirus life cycle,
and the suppression of host innate immune
responses like RNAi by B2.