Key phrases for MM1, MM2, and MM3


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MM1: Describing Materials

To Map

MM1-Step1: Detailing the source of the materials

MM1-Step1a Obtaining materials

were purchased from EtoJ

cells were purchased from

ex. cells were purchased from the American Type Culture Collection

mice were purchased from

ex. mice were purchased from the Jackson Laboratory

were obtained from EtoJ

cells were obtained from EtoJ

ex. cells were obtained from the American Type Culture Collection

samples were obtained from EtoJ

mice were obtained from

ex. mice were obtained from the Jackson Laboratory

were obtained from patients EtoJ

were kindly provided by

was a gift from

were isolated from EtoJ

cells were isolated from EtoJ

were isolated by EtoJ

were used for EtoJ

mice were used for EtoJ

were previously described EtoJ

were described previously EtoJ

 

MM1-Step1b Generating materials

were generated by EtoJ

mice were generated by EtoJ

ex. mice were generated by crossing EtoJ

cells were generated by EtoJ

construct was generated by EtoJ

generated by PCR amplification EtoJ

were generated as described EtoJ

cells were selected with EtoJ

as previously described EtoJ

as described previously EtoJ

ex. were generated as described previously EtoJ

have been previously described EtoJ

as described above EtoJ

 

 

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MM1-Step2: Preparing materials

MM1-Step2a Maintaining/Preparing materials

were maintained in EtoJ

cells were maintained in EtoJ

ex. cells were maintained in Dulbecco's modified Eagle's medium EtoJ

ex. cells were maintained in DMEM EtoJ

lines were maintained in EtoJ

mice were maintained in EtoJ

were cultured in EtoJ

cells were cultured in EtoJ

cells were cultured in DMEM EtoJ

were grown in EtoJ

cells were grown in EtoJ

lines were grown in EtoJ

were centrifuged at EtoJ

and centrifuged at EtoJ

centrifuged at * g for * min at * °C

 

MM1-Step2b Treatment

were treated with EtoJ

mice were treated with EtoJ

cells were treated with EtoJ

were injected with EtoJ

mice were injected with EtoJ

 

 

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MM1-Step3: Describing ethics

MM1-Step3a Ethics

were approved by EtoJ

experiments were approved by EtoJ

protocols were approved by EtoJ

study was approved by EtoJ

protocols approved by EtoJ

under protocols approved by EtoJ

according to protocols approved by EtoJ

were reviewed and approved by EtoJ

approved by the institutional animal care and use committee

approved by the institutional review board EtoJ

were performed in accordance with EtoJ

ex. experiments were performed in accordance with EtoJ

were conducted in accordance with EtoJ

written informed consent was obtained from EtoJ

provided written informed consent EtoJ

ex. all participants provided written informed consent EtoJ

 

 

 

 

MM2: Describing Experimental Procedures

To Map

MM2-Step1: Detailing procedures

MM2-Step1a Sample preparation

cells were seeded in EtoJ

ex. cells were seeded in 96-well plates

cells were seeded at EtoJ

cells were plated in EtoJ

were collected by centrifugation EtoJ

cells were washed with EtoJ

cells were then washed EtoJ

were washed three times EtoJ

beads were washed three times EtoJ

were washed with PBS EtoJ

cells were resuspended in EtoJ

RNA was extracted from EtoJ

RNA was extracted with EtoJ

RNA was isolated using EtoJ

cells were lysed in EtoJ

 

MM2-Step1b Experimental process

were separated by SDS-PAGE EtoJ

cells were fixed in EtoJ

were fixed in *% paraformaldehyde EtoJ

cells were fixed with EtoJ

were fixed with *% paraformaldehyde EtoJ

cells were fixed for EtoJ

ex. cells were fixed for * min

cells were stained with EtoJ

images were acquired EtoJ

images were acquired with

images were acquired using

images were taken EtoJ

images were obtained EtoJ

images were captured EtoJ

images were recorded EtoJ

images were collected EtoJ

 

MM2-Step1c Experimental reaction

were incubated with EtoJ

cells were incubated with EtoJ

sections were incubated with EtoJ

were then incubated with EtoJ

were incubated for EtoJ

were incubated for * h at * °C

cells were incubated for EtoJ

cells were treated with EtoJ

 

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MM2-Step2: Documenting established procedures

MM2-Step2a Performing experiments

analysis was performed EtoJ

analysis was performed using EtoJ

assays were performed EtoJ

ex. assays were performed as described EtoJ

experiments were performed EtoJ

PCR was performed EtoJ

PCR was performed with

imaging was performed EtoJ

staining was performed EtoJ

measurements were performed EtoJ

sequencing was performed EtoJ

assays were carried out EtoJ

were analyzed by EtoJ

were analyzed using EtoJ

were analyzed using * software EtoJ

were analyzed with

were analyzed on

analyzed by flow cytometry EtoJ

data were analyzed EtoJ

cells were analyzed EtoJ

samples were analyzed EtoJ

as previously described EtoJ

was performed as previously described EtoJ

performed as previously described with

ex. performed as previously described with minor modifications EtoJ

as previously described using EtoJ

as described previously EtoJ

was performed as described previously EtoJ

essentially as described previously EtoJ

described in the supplemental experimental procedures EtoJ

as described above EtoJ

carried out as described EtoJ

 

 

 

 

MM3: Describing Statistics and Bioinformatics

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MM3-Step1: Describing statistical procedures

MM3-Step1a Statistical analysis

statistical analyses were performed EtoJ

statistical analyses were performed using EtoJ

all statistical analyses were performed

statistical significance was determined EtoJ

significance of differences between EtoJ

was used to compare EtoJ

was used to compare two

was used to determine EtoJ

were used to estimate EtoJ

used to calculate EtoJ

test was used to EtoJ

test was used to determine EtoJ

test was used for EtoJ

t test was used EtoJ

ex. Student's t-test was used to compare EtoJ

was calculated as EtoJ

were calculated by EtoJ

were calculated using EtoJ

values were calculated EtoJ

were adjusted for EtoJ

data were analyzed with Graphpad Prism EtoJ

at least three independent (experiments) EtoJ

P < 0.05 was considered significant EtoJ

P <0.05 was considered statistically significant EtoJ

a P value of less than 0.05 was considered EtoJ

ex. a P value less than 0.05 was considered significant EtoJ

ex. a P value less than 0.05 was considered statistically significant

 

MM3-Step1b Data presentation

data are presented as EtoJ

data are presented as mean ± SEM EtoJ

data are expressed as EtoJ

ex. data are expressed as mean ± SEM EtoJ

 

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MM3-Step2: Describing bioinformatics

MM3-Step2a Bioinformatics

reads were mapped to EtoJ

were aligned to EtoJ

ex. reads were aligned to EtoJ

were assigned to EtoJ

were annotated as EtoJ

were retrieved from EtoJ

were downloaded from EtoJ

was based on EtoJ

were normalized by EtoJ

was solved by molecular replacement EtoJ

were used to generate EtoJ

used to identify EtoJ

 

 

 

 

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