【Key phrases for MM1, MM2, and MM3】
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<MM1: Describing Materials>
<MM1-Step1: Detailing the source of the materials>
MM1-Step1a (Obtaining materials)
cells were purchased from
ex. cells were purchased from the American Type Culture Collection
mice were purchased from
ex. mice were purchased from the Jackson Laboratory
ex. cells were obtained from the American Type Culture Collection
samples were obtained from EtoJ
mice were obtained from
ex. mice were obtained from the Jackson Laboratory
were obtained from patients EtoJ
were kindly provided by
was a gift from
were previously described EtoJ
were described previously EtoJ
MM1-Step1b (Generating materials)
ex. mice were generated by crossing EtoJ
construct was generated by EtoJ
generated by PCR amplification EtoJ
were generated as described EtoJ
ex. were generated as described previously EtoJ
have been previously described EtoJ
<MM1-Step2: Preparing materials>
MM1-Step2a (Maintaining/Preparing materials)
ex. cells were maintained in Dulbecco's modified Eagle's medium EtoJ
ex. cells were maintained in DMEM EtoJ
lines were maintained in EtoJ
cells were cultured in DMEM EtoJ
centrifuged at * g for * min at * °C
MM1-Step2b (Treatment)
<MM1-Step3: Describing ethics>
MM1-Step3a (Ethics)
experiments were approved by EtoJ
protocols were approved by EtoJ
under protocols approved by EtoJ
according to protocols approved by EtoJ
were reviewed and approved by EtoJ
approved by the institutional animal care and use committee
approved by the institutional review board EtoJ
were performed in accordance with EtoJ
ex. experiments were performed in accordance with EtoJ
were conducted in accordance with EtoJ
written informed consent was obtained from EtoJ
provided written informed consent EtoJ
ex. all participants provided written informed consent EtoJ
<MM2: Describing Experimental Procedures>
<MM2-Step1: Detailing procedures>
MM2-Step1a (Sample preparation)
ex. cells were seeded in 96-well plates
were collected by centrifugation EtoJ
beads were washed three times EtoJ
were washed with PBS EtoJ
cells were resuspended in EtoJ
RNA was extracted with EtoJ
MM2-Step1b (Experimental process)
were separated by SDS-PAGE EtoJ
cells were fixed in EtoJ
were fixed in *% paraformaldehyde EtoJ
were fixed with *% paraformaldehyde EtoJ
ex. cells were fixed for * min
MM2-Step1c (Experimental reaction)
cells were incubated with EtoJ
sections were incubated with EtoJ
were incubated for * h at * °C
<MM2-Step2: Documenting established procedures>
MM2-Step2a (Performing experiments)
analysis was performed using EtoJ
ex. assays were performed as described EtoJ
experiments were performed EtoJ
measurements were performed EtoJ
were analyzed using * software EtoJ
analyzed by flow cytometry EtoJ
was performed as previously described EtoJ
performed as previously described with
ex. performed as previously described with minor modifications EtoJ
as previously described using EtoJ
was performed as described previously EtoJ
essentially as described previously EtoJ
described in the supplemental experimental procedures EtoJ
carried out as described EtoJ
<MM3: Describing Statistics and Bioinformatics>
<MM3-Step1: Describing statistical procedures>
MM3-Step1a (Statistical analysis)
statistical analyses were performed EtoJ
statistical analyses were performed using EtoJ
all statistical analyses were performed
statistical significance was determined EtoJ
significance of differences between EtoJ
test was used to determine EtoJ
ex. Student's t-test was used to compare EtoJ
data were analyzed with Graphpad Prism EtoJ
at least three independent (experiments) EtoJ
P < 0.05 was considered significant EtoJ
P <0.05 was considered statistically significant EtoJ
a P value of less than 0.05 was considered EtoJ
ex. a P value less than 0.05 was considered significant EtoJ
ex. a P value less than 0.05 was considered statistically significant
MM3-Step1b (Data presentation)
data are presented as mean ± SEM EtoJ
ex. data are expressed as mean ± SEM EtoJ
<MM3-Step2: Describing bioinformatics>
MM3-Step2a (Bioinformatics)
ex. reads were aligned to EtoJ
was solved by molecular replacement EtoJ