{"id":687,"date":"2019-01-28T11:36:44","date_gmt":"2019-01-28T02:36:44","guid":{"rendered":"http:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?page_id=687"},"modified":"2025-04-23T20:23:08","modified_gmt":"2025-04-23T11:23:08","slug":"research","status":"publish","type":"page","link":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?page_id=687","title":{"rendered":"\u7814\u7a76\u6982\u8981"},"content":{"rendered":"\n<style>\nh1.cm-entry-title{\n display:none;\n}\n<\/style>\n\n\n\n<div class=\"wp-block-cover has-custom-content-position is-position-bottom-left vk_block-margin-0--margin-top\" style=\"border-radius:10px;min-height:120px;aspect-ratio:unset;\"><img loading=\"eager\" decoding=\"async\" width=\"1652\" height=\"1239\" class=\"wp-block-cover__image-background wp-image-2786\" alt=\"\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/2019-05-29_22-03-09_085.jpeg\" style=\"object-position:51% 43%\" data-object-fit=\"cover\" data-object-position=\"51% 43%\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/2019-05-29_22-03-09_085.jpeg 1652w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/2019-05-29_22-03-09_085-300x225.jpeg 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/2019-05-29_22-03-09_085-1024x768.jpeg 1024w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/2019-05-29_22-03-09_085-768x576.jpeg 768w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/2019-05-29_22-03-09_085-1536x1152.jpeg 1536w\" sizes=\"(max-width: 1652px) 100vw, 1652px\" \/><span aria-hidden=\"true\" class=\"wp-block-cover__background has-background-dim\" style=\"background-color:#776f60\"><\/span><div class=\"wp-block-cover__inner-container is-layout-constrained wp-block-cover-is-layout-constrained\">\n<p class=\"has-text-align-left has-white-color has-text-color has-link-color has-large-font-size wp-elements-c73974702c8bb34c15ca7807db2f9d64\"><a href=\"#enviroanmental_evolutionary_genomomics\" data-type=\"internal\" data-id=\"#enviroanmental_evolutionary_genomomics\">\u751f\u7269\u306e\u74b0\u5883\u9069\u5fdc\u3068\u30b2\u30ce\u30e0\u9032\u5316<\/a><\/p>\n<\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-alpha-channel-opacity\" style=\"margin-top:0;margin-bottom:0\"\/>\n\n\n\n<div class=\"wp-block-cover has-custom-content-position is-position-bottom-left vk_block-margin-sm--margin-top\" style=\"border-radius:10px;min-height:120px;aspect-ratio:unset;\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1707\" class=\"wp-block-cover__image-background wp-image-2793\" alt=\"\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_6874-001-scaled.jpg\" data-object-fit=\"cover\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_6874-001-scaled.jpg 2560w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_6874-001-300x200.jpg 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_6874-001-1024x683.jpg 1024w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_6874-001-768x512.jpg 768w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_6874-001-1536x1024.jpg 1536w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_6874-001-2048x1365.jpg 2048w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\" \/><span aria-hidden=\"true\" class=\"wp-block-cover__background has-background-dim\" style=\"background-color:#776f60\"><\/span><div class=\"wp-block-cover__inner-container is-layout-constrained wp-block-cover-is-layout-constrained\">\n<p class=\"has-text-align-left has-white-color has-text-color has-link-color has-large-font-size wp-elements-80074287ebb5761d6f3c209e6304128b\"><a href=\"#conservation_genetics\">\u7d76\u6ec5\u5371\u60e7\u4e21\u751f\u985e\u306e\u4fdd\u5168\u907a\u4f1d\u5b66<\/a><\/p>\n<\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-alpha-channel-opacity\" style=\"margin-top:0;margin-bottom:0\"\/>\n\n\n\n<div class=\"wp-block-cover has-custom-content-position is-position-bottom-left vk_block-margin-sm--margin-top\" style=\"border-radius:10px;min-height:120px;aspect-ratio:unset;\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1707\" class=\"wp-block-cover__image-background wp-image-2796\" alt=\"\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_3824-scaled.jpg\" style=\"object-position:49% 80%\" data-object-fit=\"cover\" data-object-position=\"49% 80%\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_3824-scaled.jpg 2560w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_3824-300x200.jpg 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_3824-1024x683.jpg 1024w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_3824-768x512.jpg 768w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_3824-1536x1024.jpg 1536w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/10\/IMG_3824-2048x1365.jpg 2048w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\" \/><span aria-hidden=\"true\" class=\"wp-block-cover__background has-background-dim\" style=\"background-color:#776f60\"><\/span><div class=\"wp-block-cover__inner-container is-layout-constrained wp-block-cover-is-layout-constrained\">\n<p class=\"has-text-align-left has-white-color has-text-color has-link-color has-large-font-size wp-elements-a5891e81ec47d15fabeb8f29661caa0c\"><a href=\"#population_genomics\">\u96c6\u56e3\u907a\u4f1d\u30fb\u96c6\u56e3\u30b2\u30ce\u30e0\uff08\u30b2\u30ce\u30e0\u306e\u56fa\u6709\u5316\u3068\u7a2e\u5206\u5316\uff09<\/a><\/p>\n<\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-alpha-channel-opacity\" style=\"margin-top:0;margin-bottom:0\"\/>\n\n\n\n<div class=\"wp-block-cover is-light has-custom-content-position is-position-bottom-left vk_block-margin-sm--margin-top\" style=\"border-radius:10px;min-height:120px;aspect-ratio:unset;\"><img loading=\"lazy\" decoding=\"async\" width=\"780\" height=\"99\" class=\"wp-block-cover__image-background wp-image-2987 size-full\" alt=\"\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-2.png\" data-object-fit=\"cover\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-2.png 780w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-2-300x38.png 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-2-768x97.png 768w\" sizes=\"auto, (max-width: 780px) 100vw, 780px\" \/><span aria-hidden=\"true\" class=\"wp-block-cover__background has-background-dim\" style=\"background-color:#776f60\"><\/span><div class=\"wp-block-cover__inner-container is-layout-constrained wp-block-cover-is-layout-constrained\">\n<p class=\"has-text-align-left has-white-color has-text-color has-link-color has-large-font-size wp-elements-e3dd2e758976e40505151538a86cee99\"><a href=\"#phylogenetics\">\u5206\u5b50\u7cfb\u7d71\u30fb\u7cfb\u7d71\u5730\u7406\u5b66<\/a><\/p>\n<\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-alpha-channel-opacity\" style=\"margin-top:0;margin-bottom:0\"\/>\n\n\n\n<div class=\"wp-block-cover has-custom-content-position is-position-bottom-left vk_block-margin-sm--margin-top\" style=\"border-radius:10px;min-height:120px;aspect-ratio:unset;\"><img loading=\"lazy\" decoding=\"async\" width=\"653\" height=\"156\" class=\"wp-block-cover__image-background wp-image-2996 size-full\" alt=\"\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-5.png\" data-object-fit=\"cover\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-5.png 653w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-5-300x72.png 300w\" sizes=\"auto, (max-width: 653px) 100vw, 653px\" \/><span aria-hidden=\"true\" class=\"wp-block-cover__background has-background-dim\" style=\"background-color:#776f60\"><\/span><div class=\"wp-block-cover__inner-container is-layout-constrained wp-block-cover-is-layout-constrained\">\n<p class=\"has-text-align-left has-white-color has-text-color has-link-color has-large-font-size wp-elements-bfa7ccafec68840414c8d14e469ab9e2\"><a href=\"#molecular_ecology\">\u751f\u614b\u5b66\u7684\u7814\u7a76\u306b\u304a\u3051\u308b\u5206\u5b50\u30de\u30fc\u30ab\u30fc\u306e\u5229\u7528<\/a><\/p>\n<\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-css-opacity\" style=\"margin-top:0;margin-bottom:0\"\/>\n\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"enviroanmental_evolutionary_genomomics\"> \u25a0\u751f\u7269\u306e\u74b0\u5883\u9069\u5fdc\u3068\u30b2\u30ce\u30e0\u9032\u5316 <\/h3>\n\n\n\n<p>\u8fd1\u5e74\u306e\u6c17\u5019\u5909\u52d5\u304b\u3089\u3082\u660e\u3089\u304b\u306a\u3088\u3046\u306b\u3001\u751f\u7269\u306e\u9032\u5316\u53f2\u306f\u5909\u52d5\u3059\u308b\u74b0\u5883\u3078\u306e\u9069\u5fdc\u306e\u9023\u7d9a\u3067\u3042\u3063\u305f\u3053\u3068\u306f\u9593\u9055\u3044\u3042\u308a\u307e\u305b\u3093\u3002\u307e\u305f\u540c\u6642\u306b\u305d\u308c\u305e\u308c\u306e\u751f\u7269\u3067\u72ec\u81ea\u306e\u6226\u7565\u3067\u305d\u308c\u3089\u306e\u5909\u52d5\u3092\u4e57\u308a\u8d8a\u3048\u305f\u7d50\u679c\u3001\u56fa\u6709\u306a\u751f\u614b\u3092\u7372\u5f97\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002\u30b2\u30ce\u30e0\u60c5\u5831\u304c\u6bd4\u8f03\u7684\u7c21\u5358\u306b\u624b\u306b\u5165\u308c\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u3063\u305f\u4eca\u3001\u7a2e\u751f\u614b\u304b\u3089\u30b2\u30ce\u30e0\u307e\u3067\u3001\u5e45\u5e83\u3044\u30a2\u30d7\u30ed\u30fc\u30c1\u306b\u3088\u3063\u3066\u3001\u751f\u7269\u9032\u5316\u306e\u5b9f\u614b\u3092\u8ffd\u7a76\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u69d8\u3005\u306a\u74b0\u5883\u306b\u751f\u606f\u3059\u308b\u751f\u7269\u306e\u56fa\u6709\u6027\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u73fe\u72b6\u3067\u53ef\u80fd\u306a\u9650\u308a\u5148\u7aef\u7684\u306a\u30c4\u30fc\u30eb\u3092\u7528\u3044\u3066\u3001\u305d\u308c\u3089\u306e\u907a\u4f1d\u7684\u57fa\u76e4\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002<br>\u3000\u7279\u306b\u6700\u8fd1\u306f\u3001\u6e29\u6cc9\u306b\u751f\u606f\u3059\u308b\u3068\u3044\u3046\u7279\u7570\u306a\u751f\u614b\u3092\u6709\u3057\u305f\u30ea\u30e5\u30a6\u30ad\u30e5\u30a6\u30ab\u30b8\u30ab\u30ac\u30a8\u30eb\uff08<em>Buergeria&nbsp;japonica<\/em>)\u3092\u5bfe\u8c61\u306b\u3057\u3066\u9ad8\u6e29\u9069\u5fdc\u306e\u907a\u4f1d\u7684\u57fa\u76e4\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u7814\u7a76\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"959\" height=\"640\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31.png\" alt=\"\" class=\"wp-image-715\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31.png 959w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31-300x200.png 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31-768x513.png 768w\" sizes=\"auto, (max-width: 959px) 100vw, 959px\" \/><\/figure>\n\n\n\n<p class=\"has-light-gray-background-color has-background has-small-font-size\">[\u4e3b\u8981\u95a2\u9023\u8ad6\u6587]<br>\u30fb\u4e95\u5ddd \u6b66, \u5c0f\u5dfb\u7fd4\u5e73, \u837b\u91ce \u8087 \u300c\u6e29\u6cc9\u306b\u751f\u304d\u308b\u30c9\u6839\u6027\u30ac\u30a8\u30eb\u2015\u30ea\u30e5\u30a6\u30ad\u30e5\u30a6\u30ab\u30b8\u30ab\u30ac\u30a8\u30eb\u300d\u5b9f\u9a13\u533b\u5b66 Vol.36 No.16\uff082018\u5e7410\u6708\u53f7\uff09<br>\u30fbKomaki S, Lin S-M, Nozawa M, Oumi S, Sumida M,&nbsp;Igawa T*. Fine-scale demographic processes resulting from multiple overseas colonization events of the Japanese stream tree frog,&nbsp;<em>Buergeria japonica<\/em>.&nbsp;<em>Journal of Biogeography<\/em>. 44: 1586-1597 July 2017. [&nbsp;<a href=\"http:\/\/dx.doi.org\/10.1111\/jbi.12922\/\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>&nbsp;|&nbsp;<a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jbi.12922\/abstract\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=34\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>&nbsp;] <br>\u30fbKomaki S, Lau Q, and&nbsp;Igawa T. Living in a Japanese onsen: field observations and physiological measurements of hot spring amphibian tadpoles,&nbsp;<em>Buergeria japonica<\/em>.<em>Amphibia-Reptilia<\/em>. 37: 311-314 September 2016. [&nbsp;<a href=\"http:\/\/dx.doi.org\/10.1163\/15685381-00003052\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>&nbsp;|&nbsp;<a href=\"http:\/\/booksandjournals.brillonline.com\/content\/journals\/10.1163\/15685381-00003052\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=32\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>&nbsp;] <br>\u30fbKomaki S,&nbsp;Igawa T*, Lin S-M, Sumida M. Salinity and thermal tolerance of Japanese stream tree frog (<em>Buergeria japonica<\/em>) tadpoles from island populations.<em>Herpetological Journal<\/em>. 26(3): 207-211 July 2016. [ DOI |&nbsp;<a href=\"http:\/\/www.ingentaconnect.com\/content\/bhs\/thj\/2016\/00000026\/00000003\/art00003?\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=28\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>&nbsp;]<\/p>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-css-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"conservation_genetics\">\u25a0\u7d76\u6ec5\u5371\u60e7\u4e21\u751f\u985e\u306e\u4fdd\u5168\u907a\u4f1d\u5b66<\/h3>\n\n\n\n<p>\u3000\u4e21\u751f\u985e\u306f\u305d\u306e\u540d\u306e\u3068\u304a\u308a\u3001\u9678\u57df\u3068\u6c34\u57df\u306e\u4e21\u65b9\u306e\u74b0\u5883\u306b\u4f9d\u5b58\u3057\u3001\u304b\u3064\u305d\u308c\u3089\u306e\u751f\u614b\u7cfb\u306e\u6804\u990a\u5faa\u74b0\u3092\u62c5\u3046\u8cb4\u91cd\u306a\u751f\u7269\u3067\u3059\u304c\u3001\u540c\u6642\u306b\u74b0\u5883\u4f9d\u5b58\u5ea6\u306e\u9ad8\u3044\u751f\u7269\u3067\u3042\u308b\u305f\u3081\u500b\u4f53\u6570\u6e1b\u5c11\u3001\u751f\u606f\u57df\u7e2e\u5c0f\u306b\u3088\u308b\u7d76\u6ec5\u30ea\u30b9\u30af\u306e\u9ad8\u3044\u751f\u7269\u3067\u3082\u3042\u308a\u307e\u3059\u3002\u751f\u7269\u304c\u7d76\u6ec5\u306b\u81f3\u308b\u904e\u7a0b\u306f\u3001\u300c\u7d76\u6ec5\u306e\u6e26\u300d\u3068\u547c\u3070\u308c\u308b\u60aa\u5faa\u74b0\u306b\u3088\u3063\u3066\u3001\u7d76\u6ec5\u306b\u81f3\u308b\u69d8\u3005\u306a\u30d5\u30a1\u30af\u30bf\u30fc\u304c\u52a0\u901f\u5ea6\u7684\u306b\u751f\u3058\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30e9\u30c1\u30a7\u30c3\u30c8\u306e\u3088\u3046\u306b\u5f8c\u623b\u308a\u3067\u304d\u306a\u3044\u5074\u9762\u3068\u3057\u3066\u907a\u4f1d\u7684\u591a\u69d8\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u96c6\u56e3\u306e\u7e2e\u5c0f\u304b\u3089\u907a\u4f1d\u7684\u591a\u69d8\u6027\u306e\u6e1b\u5c11\u3001\u500b\u4f53\u306b\u304a\u3051\u308b\u52a3\u52e2\u6709\u5bb3\u5909\u7570\u306e\u84c4\u7a4d\u306a\u3069\u3001\u9032\u5316\u7684\u53ef\u5851\u6027\u306e\u55aa\u5931\u304c\u5c06\u6765\u306b\u308f\u305f\u3063\u3066\u306e\u9577\u671f\u9593\u306e\u7d76\u6ec5\u30ea\u30b9\u30af\u3092\u5f15\u304d\u4e0a\u3052\u3066\u3057\u307e\u3046\u3053\u3068\u306f\u660e\u3089\u304b\u3067\u3059\u3002\u6211\u3005\u306e\u30b0\u30eb\u30fc\u30d7\u3067\u306f\u3001\u907a\u4f1d\u7684\u591a\u69d8\u6027\u3001\u7279\u306bMHC\u3092\u306f\u3058\u3081\u3068\u3059\u308b\u514d\u75ab\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u591a\u69d8\u6027\u3001\u52a3\u52e2\u6709\u5bb3\u5909\u7570\u306e\u5b9f\u614b\u89e3\u660e\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"822\" height=\"557\" data-id=\"716\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/ishikawa_pop_structure.png\" alt=\"\" class=\"wp-image-716\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/ishikawa_pop_structure.png 822w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/ishikawa_pop_structure-300x203.png 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2019\/02\/ishikawa_pop_structure-768x520.png 768w\" sizes=\"auto, (max-width: 822px) 100vw, 822px\" \/><figcaption class=\"wp-element-caption\"> <br>\u30a4\u30b7\u30ab\u30ef\u30ac\u30a8\u30eb\u7a2e\u7fa4\u3067\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u5bfe\u79f0\u7684\u306a\u907a\u4f1d\u7684\u96c6\u56e3\u69cb\u9020 (Igawa et al., 2013)<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"899\" data-id=\"2979\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-1024x899.png\" alt=\"\" class=\"wp-image-2979\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-1024x899.png 1024w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-300x263.png 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-768x674.png 768w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image.png 1235w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">\u30a2\u30ab\u30ac\u30a8\u30eb\u985e\u306b\u304a\u3051\u308bMHC\u907a\u4f1d\u5b50\u306e\u591a\u69d8\u6027 (Lau et al., 2023)<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<p>[\u4e3b\u8981\u95a2\u9023\u8ad6\u6587]<br>\u30fbLau Q,&nbsp;Igawa T, Komaki S, Satta Y, Expression Changes of MHC and other Immune Genes in Frog Skin during Ontogeny.&nbsp;<em>Animals&nbsp;<\/em>10: 91 January 2020. [&nbsp;<a href=\"https:\/\/doi.org\/10.3390\/ani10010091\">DOI<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/91\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/91\/pdf\">PDF<\/a>&nbsp;]<br>\u30fbLau Q,&nbsp;Igawa T, Minei R, Kosch TA, Satta Y. Transcriptome analyses of immune tissues from three Japanese frogs (genus&nbsp;<em>Rana<\/em>) reveals their utility in characterizing major histocompatibility complex class II.&nbsp;<em>BMC Genomics<\/em>&nbsp;18: 994 December 2017. [&nbsp;<a href=\"https:\/\/dx.doi.org\/10.1186%2Fs12864-017-4404-0\">DOI<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/s12864-017-4404-0\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/bmcgenomics.biomedcentral.com\/track\/pdf\/10.1186\/s12864-017-4404-0?site=bmcgenomics.biomedcentral.com\">PDF<\/a>&nbsp;]<br>\u30fbLau Q, and&nbsp;Igawa T, Komaki S, and Satta Y. Characterization of major histocompatibility complex class I genes in Japanese Ranidae frogs.&nbsp;<em>Immunogenetics<\/em>. 68:797-806 July 2016 &nbsp;[&nbsp;<a href=\"http:\/\/dx.doi.org\/10.1007\/s00251-016-0934-x\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>&nbsp;|&nbsp;<a href=\"http:\/\/link.springer.com\/article\/10.1007\/s00251-016-0934-x\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>&nbsp;|&nbsp;<a href=\"http:\/\/link.springer.com\/content\/pdf\/10.1007%2Fs00251-016-0934-x.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>&nbsp;]<br>\u30fbIgawa T, Sugawara H, Honda M, Tominaga A, Oumi S, Katsuren S, Ota H, Matsui M, Sumida M, Detecting inter- and intra-island genetic diversity: population structure of the endangered crocodile newt,&nbsp;<em>Echinotriton andersoni<\/em>, in the Ryukyus.&nbsp;<em>Conservation Genetics<\/em>&nbsp;21: 13-26 February 2020. [&nbsp;<a href=\"https:\/\/doi.org\/10.1007\/s10592-019-01219-8\">DOI<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/link.springer.com\/article\/10.1007\/s10592-019-01219-8\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLSfBDqjsGTHYaUwPfGJaMmte3QgPeQHSYB3OGJzB7qFw371vUg\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=44\">PDF<\/a>&nbsp;]<br>\u30fbIgawa T, Oumi S, Katsuren S, and Sumida M. Population structure and landscape genetics of two endangered frog species of genus&nbsp;<em>Odorrana<\/em>: different scenarios on two islands.&nbsp;<em>Heredity<\/em>, 110:46-56, January 2013. [&nbsp;<a href=\"http:\/\/dx.doi.org\/10.1038\/hdy.2012.59\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>&nbsp;|&nbsp;<a href=\"http:\/\/dx.doi.org\/10.1038\/hdy.2012.59\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=15\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>&nbsp;]\u3000<strong><a href=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1619\">\u89e3\u8aac<\/a><\/strong><br>\u30fbIgawa T, Nozawa M, Nagaoka M, Komaki S, Oumi S, Fujii T, and Sumida M. Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: A Case Study of the Endangered&nbsp;<em>Odorrana narina<\/em>&nbsp;Complex of Frogs.&nbsp;<em>Journal of Heredity<\/em>. 106:131-137 January 2015. [&nbsp;<a href=\"http:\/\/dx.doi.org\/10.1093\/jhered\/esu071\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>&nbsp;|&nbsp;<a href=\"http:\/\/jhered.oxfordjournals.org\/content\/106\/1\/131\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=20\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>&nbsp;]\u3000<strong><a href=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1627\">\u89e3\u8aac<\/a><\/strong><br><br><\/p>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-css-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"population_genomics\">\u25a0\u96c6\u56e3\u907a\u4f1d\u30fb\u96c6\u56e3\u30b2\u30ce\u30e0\uff08\u30b2\u30ce\u30e0\u306e\u56fa\u6709\u5316\u3068\u7a2e\u5206\u5316\uff09<\/h3>\n\n\n\n<p>\u4e00\u822c\u7684\u306b\u751f\u7269\u306f\u96c6\u56e3\u3068\u3057\u3066\u5b58\u5728\u3057\u3001\u9577\u3044\u5730\u7403\u306e\u6b74\u53f2\u3092\u751f\u304d\u5ef6\u3073\u308b\u305f\u3081\u306b\u306f\u96c6\u56e3\u4e2d\u306b\u69d8\u3005\u306a\u907a\u4f1d\u7684\u5f62\u8cea\u3092\u6709\u3057\u305f\u500b\u4f53\u304c\u5b58\u5728\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u907a\u4f1d\u7684\u591a\u69d8\u6027\u3068\u7d76\u6ec5\u30ea\u30b9\u30af\u306e\u95a2\u9023\u304c\u6307\u6458\u3055\u308c\u308b\u7d76\u6ec5\u5371\u60e7\u7a2e\u3092\u306f\u3058\u3081\u3068\u3057\u3066\u3001\u79fb\u5165\u7a2e\u3001\u7cfb\u7d71\u5316\u3055\u308c\u305f\u30e2\u30c7\u30eb\u52d5\u7269\u306b\u304a\u3051\u308b\u30b2\u30ce\u30e0\u591a\u69d8\u6027\u3092\u660e\u3089\u304b\u306b\u3057\u3001\u305d\u306e\u80cc\u666f\u306b\u3042\u308b\u74b0\u5883\u8981\u56e0\u3084\u4eba\u70ba\u6dd8\u6c70\u3068\u8868\u73fe\u578b\u3068\u306e\u95a2\u4fc2\u307e\u3067\u6df1\u304f\u6398\u308a\u4e0b\u3052\u3066\u7814\u7a76\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"3D Phylogenetic tree of Ishikawa&#039;s frogs in Okinawa and Amami Islands based on 12 SSR loci\" width=\"800\" height=\"450\" src=\"https:\/\/www.youtube.com\/embed\/Bzwz2XEENo4?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<p class=\"has-small-font-size\"><strong>\u30b5\u30d6\u30c6\u30fc\u30de\uff031\uff1a\u5909\u614b\u6642\u306b\u304a\u3051\u308b\u6e29\u5ea6\u30b9\u30c8\u30ec\u30b9\u3068\u8fd1\u89aa\u4ea4\u914d\u306e\u5f71\u97ff<\/strong><br><strong>\uff08\u6982\u8981\uff09\u3000<\/strong>\u8fd1\u5e74\u3001\u69d8\u3005\u306a\u751f\u7269\u7a2e\u306b\u304a\u3044\u3066\u3001\u8fd1\u89aa\u4ea4\u914d\u306e\u907a\u4f1d\u7684\u5f71\u97ff\uff08\u907a\u4f1d\u7684\u8377\u91cd\uff09\u304c\u89e3\u660e\u3055\u308c\u3066\u304d\u3066\u3044\u307e\u3059\u304c\u3001\u7279\u306b\u4e21\u751f\u985e\u306b\u304a\u3044\u3066\u306f\u3001\u91ce\u5916\u96c6\u56e3\u306e\u907a\u4f1d\u7684\u591a\u69d8\u6027\u3068\u751f\u5b58\u7387\u306e\u9593\u306b\u8ca0\u306e\u76f8\u95a2\u304c\u3042\u308b\u3053\u3068\uff08\u8fd1\u4ea4\u5f31\u52e2\uff09\u304c\u3042\u308b\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u591a\u304f\u306e\u7814\u7a76\u4f8b\u306f\u3001\u91ce\u5916\u306b\u7523\u5375\u3055\u308c\u305f\u5375\u3092\u89b3\u5bdf\u3059\u308b\u306e\u307f\u3067\u3001\u74b0\u5883\u306e\u9055\u3044\u3001\u5375\u306e\u72b6\u614b\u306e\u5dee\u7570\u3001\u89aa\u500b\u4f53\u306e\u907a\u4f1d\u7684\u306a\u7531\u6765\u306a\u3069\u3001\u53b3\u5bc6\u306a\u80cc\u666f\u306f\u4e0d\u660e\u78ba\u306a\u307e\u307e\u3067\u3059\u3002\u7279\u306b\u4e21\u751f\u985e\u3067\u306f\u3001\u4f53\u5916\u53d7\u7cbe\u3001\u5909\u614b\u3068\u3044\u3063\u305f\u767a\u751f\u306e\u91cd\u8981\u306a\u6bb5\u968e\u304c\u5916\u90e8\u74b0\u5883\u306b\u66b4\u9732\u3055\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u74b0\u5883\u8981\u56e0\u306b\u3088\u308b\u5f71\u97ff\u3082\u5927\u304d\u304f\u3001\u7d14\u7c8b\u306b\u907a\u4f1d\u7684\u5f71\u97ff\u3092\u62bd\u51fa\u3059\u308b\u3053\u3068\u306f\u975e\u5e38\u306b\u96e3\u3057\u3044\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u8fd1\u5e74\u3067\u306f\u5730\u7403\u74b0\u5883\u6e29\u6696\u5316\u306e\u50be\u5411\u306b\u3042\u308a\u3001\u591a\u304f\u306e\u4e21\u751f\u985e\u7a2e\u304c\u6e29\u5ea6\u30b9\u30c8\u30ec\u30b9\u306b\u3088\u308b\u5f71\u97ff\u3092\u53d7\u3051\u3064\u3064\u3042\u308b\u3053\u3068\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002<br>\u3000\u3053\u308c\u3089\u306e\u70b9\u306b\u3064\u3044\u3066\u3001\u672c\u7814\u7a76\u65bd\u8a2d\u3067\u306f\u3001\u9577\u5e74\u3001\u975e\u5b9f\u9a13\u52d5\u7269\u306e\u91ce\u5916\u500b\u4f53\u7fa4\u3092\u4eba\u5de5\u6388\u7cbe\u6cd5\u306b\u3088\u308a\u4ea4\u914d\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u3066\u304a\u308a\u3001\u53b3\u5bc6\u306a\u7ba1\u7406\u4e0b\u3067\u306e\u4ea4\u914d\u304c\u53ef\u80fd\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u7d76\u6ec5\u5371\u60e7\u7a2e\u306e\u7e41\u6b96\u306b\u3082\u6210\u529f\u3057\u3066\u304a\u308a\u3001\u5b50\u5b6b\u4e16\u4ee3\u540c\u58eb\u3067\u306e\u4ea4\u914d\u3082\u53ef\u80fd\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u7d76\u6ec5\u5371\u60e7\u7a2e\u306b\u304a\u3051\u308b\u907a\u4f1d\u7684\u306a\u5f71\u97ff\u306e\u7a0b\u5ea6\u3092\u89e3\u660e\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u3001\u4ed6\u7814\u7a76\u65bd\u8a2d\u3067\u306f\u96e3\u3057\u3044\u3053\u308c\u3089\u306e\u6280\u8853\u3092\u5229\u7528\u3057\u3001\u6e29\u5ea6\u30b9\u30c8\u30ec\u30b9\u306b\u3088\u308b\u5f71\u97ff\u3001\u8fd1\u89aa\u4ea4\u914d\u306b\u3088\u308b\u907a\u4f1d\u7684\u306a\u5f71\u97ff\u3092\u5747\u4e00\u306a\u6761\u4ef6\uff08Common Garden Experiment\uff09\u306b\u3088\u308a\u8abf\u67fb\u3057\u3066\u3044\u307e\u3059\u3002<br><strong>\uff08\u30ad\u30fc\u30ef\u30fc\u30c9\uff09\u3000<\/strong>\u4eba\u5de5\u6388\u7cbe\u3000\u30aa\u30bf\u30de\u30b8\u30e3\u30af\u30b7\u3000\u8fd1\u4ea4\u5f31\u52e2\u3000\u751f\u5b58\u7387<\/p>\n\n\n\n<p class=\"has-small-font-size\"><strong>\u30b5\u30d6\u30c6\u30fc\u30de\uff032: \u7279\u5b9a\u5916\u6765\u751f\u7269\u30a6\u30b7\u30ac\u30a8\u30eb\u306e\u907a\u4f1d\u7684\u591a\u69d8\u6027\u304a\u3088\u3073\u5c0e\u5165\u5727\u306b\u95a2\u3059\u308b\u7814\u7a76<\/strong><br><strong>(\u6982\u8981\uff09<\/strong>\u3000 \u30a6\u30b7\u30ac\u30a8\u30eb(<em>Lithobates catesbeianus<\/em>)\u306f\u672c\u6765\u3001\u30a2\u30e1\u30ea\u30ab\u5408\u8846\u56fd\u6771\u90e8\u3001\u30ab\u30ca\u30c0\u5357\u6771\u90e8\u3001\u30e1\u30ad\u30b7\u30b3\u5317\u6771\u90e8\u306b\u81ea\u7136\u306b\u5206\u5e03\u3057\u3066\u3044\u308b\u7a2e\u3067\u3059\u304c\u3001\u73fe\u5728\u3067\u306f\u4e16\u754c\u5404\u5730\u3078\u306e\u79fb\u5165\u304c\u78ba\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5143\u3005\u3001\u5927\u578b\u304b\u3064\u8caa\u6b32\u3067\u3042\u308b\u305f\u3081\u3001\u5728\u6765\u306e\u751f\u7269\u3092\u6355\u98df\u3057\u672c\u6765\u306e\u751f\u614b\u7cfb\u306e\u30d0\u30e9\u30f3\u30b9\u3092\u5d29\u3059\u53ef\u80fd\u6027\u304c\u61f8\u5ff5\u3055\u308c\u3066\u304a\u308a\u3001\u4e16\u754c\u306e\u4fb5\u7565\u7684\u5916\u6765\u7a2e\u30ef\u30fc\u30b9\u30c8100\u306b\u6307\u5b9a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u3001\u65e5\u672c\u3067\u306f2006\u5e74\u306b\u5916\u6765\u751f\u7269\u6cd5\u306b\u3088\u308a\u3001\u7279\u5b9a\u5916\u6765\u751f\u7269\u306b\u6307\u5b9a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br>\u3000\u751f\u7269\u7a2e\u306e\u6b74\u53f2\u3092\u8003\u3048\u305f\u5834\u5408\u3001\u5206\u5e03\u306e\u62e1\u5927\u306f\u5f53\u7136\u306e\u3053\u3068\u306a\u304c\u3089\u3001\u65b0\u305f\u306a\u5834\u6240\u3078\u306e\u4fb5\u5165\u306b\u3088\u3063\u3066\u6210\u3057\u9042\u3052\u3089\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u5168\u304f\u65b0\u3057\u3044\u5834\u6240\u3078\u306e\u4fb5\u5165\u306f\u30ea\u30b9\u30af\u304c\u4f34\u3044\u3001\u5fc5\u305a\u6210\u529f\u3059\u308b\u3068\u306f\u9650\u308a\u307e\u305b\u3093\u3002\u4fb5\u5165\u304c\u6210\u529f\u3059\u308b\u305f\u3081\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u8aac\u660e\u3059\u308b\u4eee\u8aac\u306f\u7a2e\u306b\u3088\u3063\u3066\u69d8\u3005\u3042\u308a\u3001\u4e00\u822c\u5316\u3059\u308b\u3053\u3068\u306f\u96e3\u3057\u3044\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u6563\u5e03\u4f53\u306e\u5c0e\u5165\u5727\uff08\u5c0e\u5165\u3055\u308c\u305f\u500b\u4f53\u6570\u3042\u308b\u3044\u306f\u3001\u5c0e\u5165\u56de\u6570\uff09\u306f\u3001\u6570\u5c11\u306a\u3044\u5168\u4f53\u7684\u306b\u5408\u610f\u3092\u5f97\u3089\u308c\u3066\u3044\u308b\u8981\u56e0\u306e\u4e00\u3064\u3067\u3067\u3059\u3002\u5177\u4f53\u7684\u306b\u306f\u3001\u907a\u4f1d\u7684\u30fb\u4eba\u53e3\u5b66\u7684\u80cc\u666f\u306b\u3088\u3063\u3066\u3001\u3088\u308a\u591a\u304f\u306e\u500b\u4f53\u3001\u3042\u308b\u3044\u306f\u3001\u3088\u308a\u591a\u304f\u56de\u6570\u306e\u5c0e\u5165\u30a4\u30d9\u30f3\u30c8\u3092\u7d4c\u9a13\u3057\u305f\u500b\u4f53\u7fa4\u304c\u3001\u3088\u308a\u5b9a\u7740\u3057\u6613\u3044\u3068\u3044\u3046\u8003\u3048\u65b9\u3067\u3059\u3002\u3053\u306e\u3053\u3068\u306f\u3001\u7d76\u6ec5\u5371\u60e7\u7a2e\u3068\u540c\u69d8\u306b\u907a\u4f1d\u7684\u591a\u69d8\u6027\u304c\u3001\u751f\u7269\u306e\u9032\u5316\u7684\u53ef\u5851\u6027\uff08\u96c6\u56e3\u304c\u74b0\u5883\u306b\u9069\u5fdc\u3057\u3066\u3001\u9032\u5316\u3059\u308b\u6f5c\u5728\u7684\u306a\u80fd\u529b\uff09\u3068\u5bc6\u63a5\u306b\u95a2\u4fc2\u3057\u3066\u3044\u308b\u3053\u3068\u3068\u307b\u307c\u540c\u69d8\u306e\u3053\u3068\u3067\u3042\u308a\u3001\u9650\u3089\u308c\u305f\u500b\u4f53\u6570\u304b\u3089\u5275\u59cb\u3055\u308c\u305f\u5916\u6765\u7a2e\u304c\u3069\u308c\u307b\u3069\u306e\u591a\u69d8\u6027\u3092\u4fdd\u6301\u3057\u3066\u3044\u308b\u306e\u304b\u306f\u3001\u4fdd\u5168\u907a\u4f1d\u5b66\u7684\u306a\u89b3\u70b9\u304b\u3089\u3082\u8208\u5473\u304c\u6301\u305f\u308c\u307e\u3059\u3002<br>\u3000\u73fe\u5728\u3001\u91ce\u751f\u5316\u3057\u3066\u3044\u308b\u30a6\u30b7\u30ac\u30a8\u30eb\u306e\u5927\u90e8\u5206\u306e\u7531\u6765\u306f\u30011918\u5e74\u306b\u30a2\u30e1\u30ea\u30ab\u5408\u8846\u56fd\u30eb\u30a4\u30b8\u30a2\u30ca\u5dde\u30cb\u30e5\u30fc\u30aa\u30ea\u30f3\u30ba\u304b\u3089\u8f38\u5165\u3055\u308c\u305f\u30aa\u30b912\u5339\u3068\u30e1\u30b95\u5339\u306b\u9061\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u6cb3\u91ce (1913)\u306b\u3088\u308c\u3070\u3001\u3053\u308c\u308917\u5339\u306e\u5b50\u5b6b\u306f\u8328\u6728\u770c\u304a\u3088\u3073\u3001\u6ecb\u8cc0\u770c\u6c34\u7523\u8a66\u9a13\u5834\u306b\u304a\u3044\u3066\u5927\u91cf\u7e41\u6b96\u306b\u7528\u3044\u3089\u308c\u30011930\u5e74\u307e\u3067\u306b52\u4e07\u5339\u304c\u5168\u56fd\u306b\u914d\u5e03\u3055\u308c\u305f\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u305d\u306e\u5f8c\u3001\u5404\u5730\u306e\u990a\u86d9\u5834\u306b\u304a\u3044\u3066\u3001\u4f55\u5ea6\u3082\u72ec\u81ea\u306b\u8f38\u5165\u3057\u305f\u3089\u3057\u304f(\u9577\u8c37\u5ddd 1999\u3001\u5ca9\u6fa4 1968)\u3001\u672c\u90a6\u306b\u304a\u3051\u308b\u5c0e\u5165\u500b\u4f53\u7fa4\u306e\u7531\u6765\u3001\u304a\u3088\u3073\u6709\u52b9\u96c6\u56e3\u30b5\u30a4\u30ba\u3001\u73fe\u5728\u306e\u500b\u4f53\u7fa4\u306b\u304a\u3051\u308b\u907a\u4f1d\u7684\u591a\u69d8\u6027\u306e\u5b9f\u614b\u306f\u672a\u3060\u306b\u4e0d\u660e\u3067\u3059\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u907a\u4f1d\u7684\u6307\u6a19\u3092\u7528\u3044\u3066\u3001\u5c0e\u5165\u500b\u4f53\u306e\u7531\u6765\u30a6\u30b7\u30ac\u30a8\u30eb\u306e\u4fb5\u5165\u6210\u529f\u30d7\u30ed\u30bb\u30b9\u306b\u304a\u3051\u308b\u5c0e\u5165\u5727\u3092\u691c\u8a3c\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u7814\u7a76\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<br><strong>\uff08\u30ad\u30fc\u30ef\u30fc\u30c9\uff09<\/strong>\u3000\u4fb5\u5165\u6210\u529f\u3000\u79fb\u5165\u3000\u8fd1\u4ea4\u5f31\u52e2 <\/p>\n\n\n\n<p class=\"has-light-gray-background-color has-background has-small-font-size\">[\u4e3b\u8981\u95a2\u9023\u8ad6\u6587]<br> <br>\u30fbMatsunami M,\u00a0Igawa T, Michimae H, Miura T, and Nishimura K. Population structure and evolution after speciation of the Hokkaido salamander (<em>Hynobius retardatus<\/em>).\u00a0<em>PLoS One<\/em>. 11(6): e0156815 June 2016. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1371\/journal.pone.0156815\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/journals.plos.org\/plosone\/article?id=10.1371%2Fjournal.pone.0156815\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"http:\/\/journals.plos.org\/plosone\/article\/asset?id=10.1371\/journal.pone.0156815.PDF\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0] <br>\u30fb Sultana N\u2020,\u00a0Igawa T\u2020*, Islam MM, Hasan M, Alam MS, Komaki S, Kawamura K, Khan MMR, and Sumida M. Inter- and intra-specific genetic divergence of Asian tiger frogs (genus\u00a0<em>Hoplobatrachus<\/em>), with special reference to the population structure of\u00a0<em>H. tigerinus<\/em>\u00a0in Bangladesh.\u00a0<em>Genes &amp; Genetic Systems<\/em>. 91: 217-228 August 2016. (\u2020 The first two authors contributed equally to the study.) [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1266\/ggs.16-00006\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"https:\/\/www.jstage.jst.go.jp\/article\/ggs\/91\/4\/91_16-00006\/_html\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=29\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0] <br>\u30fbIgawa T*, Watanabe A, Suzuki A, Kashiwagi A, Kashiwagi K, Noble A, Guille M, Simpson DE, Horb ME, Fujii T, and Sumida M. Inbreeding ratio and genetic relationships among strains of the Western clawed frog,\u00a0<em>Xenopus tropicalis<\/em>.\u00a0<em>PLoS One<\/em>. 10(7): e0133963 July 2015. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1371\/journal.pone.0133963\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0133963\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"http:\/\/journals.plos.org\/plosone\/article\/asset?id=10.1371\/journal.pone.0133963.PDF\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0] <br>\u30fb Igawa T*, Nozawa M, Nagaoka M, Komaki S, Oumi S, Fujii T, and Sumida M. Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: A Case Study of the Endangered\u00a0<em>Odorrana narina<\/em>\u00a0Complex of Frogs.\u00a0<em>Journal of Heredity<\/em>. 106:131-137 January 2015. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1093\/jhered\/esu071\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/jhered.oxfordjournals.org\/content\/106\/1\/131\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=20\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0] <br>\u30fbIgawa T*, Oumi S, Katsuren S, and Sumida M. Population structure and landscape genetics of two endangered frog species of genus\u00a0<em>Odorrana<\/em>: different scenarios on two islands.\u00a0<em>Heredity<\/em>, 110:46-56, January 2013. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1038\/hdy.2012.59\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/dx.doi.org\/10.1038\/hdy.2012.59\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=15\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0] <br>\u30fbKakehashi R,\u00a0Igawa T, Iwai N, Shoda-Kagaya E, and Sumida M. Development and characterization of new microsatellite loci in the Otton frog (<em>Babina subaspera<\/em>) and cross-amplification in a congeneric species, Holst\u2019s frog (<em>B. holsti<\/em>).\u00a0<em>Conservation Genetics Resources<\/em>, 5(4):1071-1073, June 2013. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1007\/s12686-013-9981-x\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/link.springer.com\/10.1007\/s12686-013-9981-x\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=13\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0] <\/p>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-css-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"phylogenetics\">\u25a0 \u5206\u5b50\u7cfb\u7d71\u30fb\u7cfb\u7d71\u5730\u7406\u5b66\u7684\u7814\u7a76 <\/h3>\n\n\n\n<p>\u3000 \u4e21\u751f\u985e\u306f\u6c34\u4e2d\u751f\u6d3b\u306e\u9b5a\u985e\u304b\u3089\u9678\u306b\u4e0a\u9678\u3057\u305f\u6700\u521d\u306e\u810a\u690e\u52d5\u7269\u3067\u3042\u308a\u30013\u5104\u5e74\u4ee5\u4e0a\u306e\u53e4\u3044\u8d77\u6e90\u3092\u6301\u3064\u52d5\u7269\u3067\u3059\u3002\u305d\u308c\u3086\u3048\u3001\u5f62\u614b\u3084\u751f\u614b\u3082\u591a\u69d8\u306b\u9032\u5316\u3057\u3066\u3044\u307e\u3059\u3002\u79c1\u304c\u5b66\u751f\u3068\u3057\u3066\u7814\u7a76\u3092\u59cb\u3081\u305f\u5f53\u521d\u306f\u3001\u65e5\u672c\u306e\u672c\u5dde\u306e\u4e21\u751f\u985e\u306b\u3064\u3044\u3066\u3001\u672c\u5dde\u5185\u3067\u3082\u7cfb\u7d71\u7684\u304c\u7570\u306a\u308b\u96c6\u56e3\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u306f\u5206\u304b\u3063\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u305d\u308c\u3089\u304c\u3069\u306e\u3050\u3089\u3044\u306e\u5e74\u4ee3\u3067\u5206\u5c90\u3057\u3001\u3069\u306e\u3088\u3046\u306a\u53e4\u5730\u7406\u5b66\u7684\u30a4\u30d9\u30f3\u30c8\u306b\u3088\u3063\u3066\u5f15\u304d\u8d77\u3053\u3055\u308c\u305f\u306e\u304b\u5206\u304b\u3063\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u305d\u3053\u3067\u3001\u30d2\u30ad\u30ac\u30a8\u30eb\u985e\u3092\u5bfe\u8c61\u3068\u3057\u3066\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u907a\u4f1d\u5b50\u3092\u7528\u3044\u3066\u5206\u5c90\u5e74\u4ee3\u89e3\u6790\u3092\u884c\u3044\u3001\u5730\u7406\u5b66\u5206\u91ce\u306e\u7db2\u7f85\u7684\u306a\u6587\u732e\u691c\u7d22\u304b\u3089\u3001\u897f\u65e5\u672c\u3068\u6771\u65e5\u672c\u306e\u30cb\u30db\u30f3\u30d2\u30ad\u30ac\u30a8\u30eb\u3068\u30a2\u30ba\u30de\u30d2\u30ad\u30ac\u30a8\u30eb\u306e\u5206\u5316\u306b\u3064\u3044\u3066\u3001\u5206\u5c90\u5e74\u4ee3\u3068\u305d\u308c\u3092\u5f15\u304d\u8d77\u3053\u3057\u305f\u53e4\u5730\u7406\u5b66\u7684\u30a4\u30d9\u30f3\u30c8\u3092\u89e3\u660e\u3057\u307e\u3057\u305f(Igawa et al. 2006)\u3002\u305d\u308c\u4ee5\u964d\u3001\u69d8\u3005\u306a\u52d5\u7269\u306e\u5927\u7cfb\u7d71\u30fb\u7a2e\u5206\u5316\u306b\u95a2\u3059\u308b\u7814\u7a76\u3092\u624b\u639b\u3051\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"856\" height=\"624\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-3.png\" alt=\"\" class=\"wp-image-2989\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-3.png 856w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-3-300x219.png 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-3-768x560.png 768w\" sizes=\"auto, (max-width: 856px) 100vw, 856px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"604\" height=\"623\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-4.png\" alt=\"\" class=\"wp-image-2990\" style=\"width:385px;height:auto\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-4.png 604w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-4-291x300.png 291w\" sizes=\"auto, (max-width: 604px) 100vw, 604px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p>[\u4e3b\u8981\u95a2\u9023\u8ad6\u6587]<br>\u30fbIgawa T, Nozawa M, Suzuki DG, Reimer JD, Morov AR, Wang Y, Henmi Y, Yasui K.\u00a0Evolutionary history of the extant amphioxus lineage with shallow-branching diversification.\u00a0<em>Scientific Reports<\/em>. 7: 1157 April 2017. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1038\/s41598-017-00786-5\">DOI<\/a>\u00a0|\u00a0<a href=\"https:\/\/www.nature.com\/articles\/s41598-017-00786-5\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/www.nature.com\/articles\/s41598-017-00786-5\">PDF<\/a>\u00a0]<br>\u30fbIgawa T, Kurabayashi A, Usuki C, Fujii T, and *Sumida M. Complete mitochondrial genomes of three neobatrachian anurans: A case study of divergence time estimation using different data and calibration settings.\u00a0<em>Gene<\/em>, 407(1-2):116-129, January 2008. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.gene.2007.10.001\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0378111907005082\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=3\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0]<br>\u30fbIgawa T, Kurabayashi A, Nishioka M, and *Sumida M. Molecular phylogenetic relationship of toads distributed in the Far East and Europe inferred from the nucleotide sequences of mitochondrial DNA genes.\u00a0<em>Molecular phylogenetics and evolution<\/em>, 38(1):250-60, January 2006. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.ympev.2005.09.003\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.ympev.2005.09.003%20http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16266814\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=1\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0]<\/p>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-css-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"molecular_ecology\">\u25a0\u751f\u614b\u5b66\u7684\u7814\u7a76\u306b\u304a\u3051\u308b\u5206\u5b50\u30de\u30fc\u30ab\u30fc\u306e\u5229\u7528<\/h3>\n\n\n\n<p>\u3000\u7cfb\u7d71\u89e3\u6790\u3084\u96c6\u56e3\u907a\u4f1d\u89e3\u6790\u304b\u3089\u5f97\u3089\u308c\u305f\u60c5\u5831\u3092\u4f7f\u3063\u3066\u3001\u74b0\u5883DNA\u3084\u3001\u7523\u5730\u304c\u4e0d\u660e\u306a\u500b\u4f53\u306e\u907a\u4f1d\u5b50\u9451\u5b9a\u306a\u3069\u306e\u6280\u8853\u958b\u767a\u3068\u751f\u614b\u5b66\u7684\u7814\u7a76\u3078\u306e\u5fdc\u7528\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"822\" height=\"423\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-6.png\" alt=\"\" class=\"wp-image-2998\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-6.png 822w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-6-300x154.png 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2025\/04\/image-6-768x395.png 768w\" sizes=\"auto, (max-width: 822px) 100vw, 822px\" \/><figcaption class=\"wp-element-caption\">\u74b0\u5883DNA\u3092PCR-RFLP\u6cd5\u3092\u7528\u3044\u3066\u691c\u51fa\u3059\u308b\u5b9f\u9a13\u7cfb\u306e\u4e00\u4f8b (Igawa et al., 2019)<\/figcaption><\/figure>\n\n\n\n<p>[\u4e3b\u8981\u95a2\u9023\u8ad6\u6587]<br>\u30fbOgata S, Doi H,\u00a0Igawa T, Komaki S, *Takahara T, Environmental DNA methods for detecting two invasive alien species (American bullfrog and red swamp crayfish) in Japanese ponds.\u00a0<em>Ecology Research<\/em>\u00a037: 701-710 Nov 2022 [\u00a0<a href=\"http:\/\/doi.org\/10.1111\/1440-1703.12341\">DOI<\/a>\u00a0|\u00a0<a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1703.12341\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLSfBDqjsGTHYaUwPfGJaMmte3QgPeQHSYB3OGJzB7qFw371vUg\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=58\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0]<br>\u30fbTakahara T, Iwai N, Yasumiba K,\u00a0Igawa T, Comparison of the detection of 3 endangered frog species by eDNA and acoustic surveys across three seasons.\u00a0<em>Freshwater Science<\/em>\u00a0 39: 18-27 February 2020. [\u00a0<a href=\"https:\/\/doi.org\/10.1086\/707365\">DOI<\/a>\u00a0|\u00a0<a href=\"https:\/\/www.journals.uchicago.edu\/doi\/full\/10.1086\/707365\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLSfBDqjsGTHYaUwPfGJaMmte3QgPeQHSYB3OGJzB7qFw371vUg\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=45\">PDF<\/a>\u00a0]<br>\u30fbIgawa T, *Takahara T, Lau Q, Komaki S, An application of PCR-RFLP species identification assay for environmental DNA detection.\u00a0<em>PeerJ\u00a0<\/em>7: e7597 October 2019. [\u00a0<a href=\"https:\/\/doi.org\/10.7717\/peerj.7597\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"https:\/\/peerj.com\/articles\/7597\/\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/peerj.com\/articles\/7597.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0]\u00a0<br>\u30fbIgawa T, Komaki S, Takahara T, and Sumida M. Development and Validation of PCR-RFLP Assay to Identify Three Japanese Brown Frogs of The True Frog Genus\u00a0<em>Rana<\/em>.\u00a0<em>Current Hepetology<\/em>. 34: 89-94 February 2015. \u00a0[\u00a0<a href=\"http:\/\/dx.doi.org\/10.5358\/hsj.34.89\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/www.bioone.org\/doi\/full\/10.5358\/hsj.34.89\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=21\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0]<\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u25a0\u751f\u7269\u306e\u74b0\u5883\u9069\u5fdc\u3068\u30b2\u30ce\u30e0\u9032\u5316 \u8fd1\u5e74\u306e\u6c17\u5019<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"colormag_page_container_layout":"default_layout","colormag_page_sidebar_layout":"default_layout","_locale":"ja","_original_post":"687","footnotes":""},"class_list":["post-687","page","type-page","status-publish","hentry","ja"],"_links":{"self":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/pages\/687","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=687"}],"version-history":[{"count":41,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/pages\/687\/revisions"}],"predecessor-version":[{"id":2999,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/pages\/687\/revisions\/2999"}],"wp:attachment":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}