{"id":1145,"date":"2019-12-24T00:24:17","date_gmt":"2019-12-23T15:24:17","guid":{"rendered":"http:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1145"},"modified":"2022-01-23T20:34:04","modified_gmt":"2022-01-23T11:34:04","slug":"bioinfonanopore-read%e3%82%92%e3%83%95%e3%82%a3%e3%83%ab%e3%82%bf%e3%83%aa%e3%83%b3%e3%82%b0%e3%81%99%e3%82%8b","status":"publish","type":"post","link":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1145","title":{"rendered":"[bioinfo]Nanopore read\u3092\u30d5\u30a3\u30eb\u30bf\u30ea\u30f3\u30b0\u3059\u308b"},"content":{"rendered":"\n<p>Nanopore\u306e\u30c7\u30fc\u30bf\u306f\u305d\u306e\u307e\u307e\u3067\u306f\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306elambda phage\u30b2\u30ce\u30e0\u306a\u3069\u3092\u542b\u3093\u3060\u307e\u307e\u3067\u3001\u30a2\u30bb\u30f3\u30d6\u30ea\u7b49\u306b\u4f7f\u3046\u306b\u306f\u305d\u308c\u3089\u304c\u90aa\u9b54\u3092\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002\u305d\u3053\u3067\u3001Nanopack\u30d1\u30c3\u30b1\u30fc\u30b8\u306e\u4e2d\u306eNanoLyse\u3001Nanoflit\u3092\u4f7f\u3063\u3066\u30ea\u30fc\u30c9\u3092\u30d5\u30a3\u30eb\u30bf\u30ea\u30f3\u30b0\u3059\u308b\u3002<\/p>\n<p>Nanopack\u306fpip\u304b\u3089\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u53ef\u80fd\u3002<\/p>\n\n\n\n<pre class=\"wp-block-code\"><code>pip install nanopack<\/code><\/pre>\n\n\n\n<p>guppy\u3092\u4f7f\u3063\u3066\u30b3\u30fc\u30eb\u3057\u305fNanopore\u306efastq\u30c7\u30fc\u30bf\u3092cat\u3067\u6d41\u3057\u8fbc\u3080\u3002<\/p>\n\n\n\n<pre class=\"wp-block-code\"><code>cat *.fastq | NanoLyse  --reference lambda.fa.gz| NanoFilt -q 10 | gzip > trimmed_filtered.fq.gz<\/code><\/pre>\n\n\n\n<pre class=\"wp-block-code\"><code><\/code><\/pre>\n\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"colormag_page_container_layout":"default_layout","colormag_page_sidebar_layout":"default_layout","_locale":"ja","_original_post":"1145","footnotes":""},"categories":[7],"tags":[],"class_list":["post-1145","post","type-post","status-publish","format-standard","hentry","category-protocol","ja"],"_links":{"self":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1145","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1145"}],"version-history":[{"count":4,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1145\/revisions"}],"predecessor-version":[{"id":1699,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1145\/revisions\/1699"}],"wp:attachment":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1145"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1145"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1145"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}