{"id":1354,"date":"2020-07-18T01:37:14","date_gmt":"2020-07-17T16:37:14","guid":{"rendered":"http:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1354"},"modified":"2020-07-18T01:37:17","modified_gmt":"2020-07-17T16:37:17","slug":"atbenchnanopore-sequeincing-sqk-lsk109%e3%81%ab%e7%94%a8%e3%81%84%e3%82%8bneb-quick-t4-ligase%e3%81%ae%e3%82%ad%e3%83%83%e3%83%88%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6","status":"publish","type":"post","link":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1354","title":{"rendered":"[atbench]Nanopore Sequeincing, SQK-LSK109\u306b\u7528\u3044\u308bNEB Quick T4 Ligase\u306e\u30ad\u30c3\u30c8\u306b\u3064\u3044\u3066"},"content":{"rendered":"\n<p>Nanopore\u306eLigation sequence SQK-LSK109\u3067\u306f\u30e9\u30a4\u30d6\u30e9\u30ea\u4f5c\u6210\u306e\u969b\u306bNEB\u306eQuick T4 Ligase\u309210 uL\u3082\u6d88\u8cbb\u3059\u308b\u3002ONT\u3067\u306fNEB\u306eE6056 NEBNext Quick Ligation Module\u3092\u6307\u5b9a\u3057\u3066\u3044\u308b\u304c\u3001\u5b9f\u969b\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f\u4f7f\u7528\u3059\u308b\u306e\u306fQuick T4 Ligase\u3060\u3051\u3067\u3042\u308a\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u4f7f\u308f\u306a\u3044\u3002\u307e\u305f\u3053\u306eQuick T4 Ligase\u304c\u5165\u3063\u305fNEB\u306e\u30ad\u30c3\u30c8\u306f\u8907\u6570\u3042\u308a\u3001M2200 Quick Ligation Kit\u304c\u30bb\u30fc\u30eb\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u306f\u5272\u5b89\u3067\u3042\u308b\u3053\u3068\u306b\u6c17\u3065\u3044\u305f\u3002<\/p>\n<p>\u6c17\u3092\u3064\u3051\u306a\u3051\u308c\u3070\u3044\u3051\u306a\u3044\u306e\u306f\u5404\u30ad\u30c3\u30c8\u3067\u4f7f\u7528\u53ef\u80fd\u56de\u6570\u3092\u66f8\u3044\u3066\u3044\u308b\u304c\u3001\u4e0b\u8a18\u306eURL\u306e\u901a\u308a\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\u9055\u3046\u306e\u3067\u5b9f\u969b\u306e\u4fa1\u683c\u306f\u305d\u308c\u307b\u3069\u9055\u308f\u306a\u3044\u70b9\u3067\u3042\u308b\u3002<\/p>\n<p><a href=\"https:\/\/international.neb.com\/protocols\/0001\/01\/01\/nebnext-quick-ligation-module-protocol-e6056\">https:\/\/international.neb.com\/protocols\/0001\/01\/01\/nebnext-quick-ligation-module-protocol-e6056<\/a><\/p>\n<p><a href=\"https:\/\/international.neb.com\/protocols\/0001\/01\/01\/quick-ligation-protocol\">https:\/\/international.neb.com\/protocols\/0001\/01\/01\/quick-ligation-protocol<\/a><\/p>\n<p>\u4e00\u3064\u4e00\u3064\u66f8\u304f\u3068\u9762\u5012\u306a\u306e\u3067\u4e0b\u8a18\u306e\u30c6\u30fc\u30d6\u30eb\u306b\u307e\u3068\u3081\u305f\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td>product<\/td>\n<td>price<\/td>\n<td>vol. in kit protocol<\/td>\n<td>number of reaction in kit protocol<\/td>\n<td>total vol.<\/td>\n<td>price\/10 uL<\/td>\n<\/tr>\n<tr>\n<td>E5056S<\/td>\n<td>\u00a546,200<\/td>\n<td>5<\/td>\n<td>20<\/td>\n<td>100<\/td>\n<td>\u00a54,620\u00a0<\/td>\n<\/tr>\n<tr>\n<td>E5056L<\/td>\n<td>\u00a5184,800<\/td>\n<td>5<\/td>\n<td>100<\/td>\n<td>500<\/td>\n<td>\u00a53,696<\/td>\n<\/tr>\n<tr>\n<td>M2200S<\/td>\n<td><s>\u00a518,700<\/s><br \/>\u00a514,025<\/td>\n<td>1<\/td>\n<td>30<\/td>\n<td>30<\/td>\n<td><s>\u00a56,233<\/s>\u00a54,675<\/td>\n<\/tr>\n<tr>\n<td>M2200L<\/td>\n<td><s>\u00a574,800<\/s><br \/>\u00a556,100<\/td>\n<td>1<\/td>\n<td>150<\/td>\n<td>150<\/td>\n<td><s>\u00a54,987<\/s>\u00a53,740<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u7d50\u5c40\u3001\u4eca\u56de\u306fM2200L\u3092\u8cfc\u5165\u3057\u305f\uff01<\/p>\n<p>Nanopore\u306e\u30b3\u30df\u30e5\u30cb\u30c6\u30a3\u306b\u3082\u30b3\u30e1\u30f3\u30c8\u3059\u308b\u5f62\u3067\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u8a18\u3057\u3066\u304a\u3044\u305f\u3002<\/p>\n<p>I just found M2200S and M2200L are in sale now (2020\/07\/17) and so compared the cost performance. In Japan E6056S and E5056L (not T) are only available.<\/p>\n<p>According to NEB, T4 ligase itself kits are completely same in these kits, but number of reaction and its standard protocol are different between those. In each standard protocol, T4 ligase is recommend to be used 5 uL and 1 uL in E6056 and M2200.<\/p>\n<p><a href=\"https:\/\/international.neb.com\/protocols\/0001\/01\/01\/nebnext-quick-ligation-module-protocol-e6056\">https:\/\/international.neb.com\/protocols\/0001\/01\/01\/nebnext-quick-ligation-module-protocol-e6056<\/a><\/p>\n<p><a href=\"https:\/\/international.neb.com\/protocols\/0001\/01\/01\/quick-ligation-protocol\">https:\/\/international.neb.com\/protocols\/0001\/01\/01\/quick-ligation-protocol<\/a><\/p>\n<p>This could be summarized following table:<\/p>\n<table>\n<tbody>\n<tr>\n<td>product<\/td>\n<td>price<\/td>\n<td>vol. in kit protocol<\/td>\n<td>number of reaction in kit protocol<\/td>\n<td>total vol.<\/td>\n<td>price\/10 uL<\/td>\n<\/tr>\n<tr>\n<td>E5056S<\/td>\n<td>\u00a546,200<\/td>\n<td>5<\/td>\n<td>20<\/td>\n<td>100<\/td>\n<td>\u00a54,620\u00a0<\/td>\n<\/tr>\n<tr>\n<td>E5056L<\/td>\n<td>\u00a5184,800<\/td>\n<td>5<\/td>\n<td>100<\/td>\n<td>500<\/td>\n<td>\u00a53,696<\/td>\n<\/tr>\n<tr>\n<td>M2200S<\/td>\n<td><s>\u00a518,700<\/s><br \/>\u00a514,025<\/td>\n<td>1<\/td>\n<td>30<\/td>\n<td>30<\/td>\n<td><s>\u00a56,233<\/s>\u00a54,675<\/td>\n<\/tr>\n<tr>\n<td>M2200L<\/td>\n<td><s>\u00a574,800<\/s><br \/>\u00a556,100<\/td>\n<td>1<\/td>\n<td>150<\/td>\n<td>150<\/td>\n<td><s>\u00a54,987<\/s>\u00a53,740<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Considering upfront price and expiration time, I bought M2200L this time!<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"colormag_page_container_layout":"default_layout","colormag_page_sidebar_layout":"default_layout","_locale":"ja","_original_post":"http:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1354","footnotes":""},"categories":[7],"tags":[],"class_list":["post-1354","post","type-post","status-publish","format-standard","hentry","category-protocol","ja"],"_links":{"self":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1354","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1354"}],"version-history":[{"count":1,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1354\/revisions"}],"predecessor-version":[{"id":1355,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1354\/revisions\/1355"}],"wp:attachment":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1354"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1354"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1354"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}