{"id":1627,"date":"2021-09-12T18:02:28","date_gmt":"2021-09-12T09:02:28","guid":{"rendered":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1627"},"modified":"2021-09-12T18:02:32","modified_gmt":"2021-09-12T09:02:32","slug":"%e8%ab%96%e6%96%87%e8%a7%a3%e8%aa%ac%ef%bc%9amicrosatellite-marker-development-by-multiplex-ion-torrent-pgm-sequencing-a-case-study-of-the-endangered-odorrana-narina-complex-of-frogs","status":"publish","type":"post","link":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1627","title":{"rendered":"\u8ad6\u6587\u89e3\u8aac\uff1aMicrosatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: A Case Study of the Endangered Odorrana narina Complex of Frogs."},"content":{"rendered":"\n<p>*Igawa T, Nozawa M, Nagaoka M, Komaki S, Oumi S, Fujii T, and Sumida M. Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: A Case Study of the Endangered <em>Odorrana narina<\/em> Complex of Frogs. <em>Journal of Heredity<\/em>. 106:131-137 January 2015. [\u00a0<a href=\"http:\/\/dx.doi.org\/10.1093\/jhered\/esu071\" target=\"_blank\" rel=\"noreferrer noopener\">DOI<\/a>\u00a0|\u00a0<a href=\"http:\/\/jhered.oxfordjournals.org\/content\/106\/1\/131\" target=\"_blank\" rel=\"noreferrer noopener\">http<\/a>\u00a0|\u00a0<a href=\"https:\/\/docs.google.com\/forms\/d\/1IY6AU87dR2cY1JIPlPQNcfxTeF6VQjvIeFaK3Yu5vrc\/viewform?entry.1456703309&amp;entry.1617305678&amp;entry.110948457=20\" target=\"_blank\" rel=\"noreferrer noopener\">PDF<\/a>\u00a0]<\/p>\n\n\n\n<p>\u3000\u672c\u7814\u7a76\u3067\u306f\u3001\u96c6\u56e3\u907a\u4f1d\u5b66\u7684\u89e3\u6790\u306b\u304a\u3044\u3066\u6709\u7528\u306a\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u30de\u30fc\u30ab\u30fc\u306e\u5bfe\u8c61\u7a2e\u30b2\u30ce\u30e0\u304b\u3089\u306e\u5358\u96e2\u65b9\u6cd5\u306b\u304a\u3044\u3066\u3001\u8fd1\u5e74\u3001\u9032\u5c55\u306e\u8457\u3057\u3044\u6280\u8853\u3067\u3042\u308b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u3092\u5c0e\u5165\u3057\u3001\u5b89\u4fa1\u306b\u304b\u3064\u3001\u8fc5\u901f\u306b\u884c\u3046\u65b9\u6cd5\u3092\u958b\u767a\u3057\u305f\u3002<\/p>\n\n\n\n<p>\u3000\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u9818\u57df\u306f\u3001\u30b2\u30ce\u30e0\u4e2d\u306b\u6563\u5728\u3059\u308b\u30b7\u30f3\u30d7\u30eb\u306a\u7e70\u308a\u8fd4\u3057\u914d\u5217\uff08\u4f8b\uff1aATATATATAT\uff09\u3067\u3042\u308b\u3002DNA\u8907\u88fd\u6642\u306e\u30b3\u30d4\u30fc\u30df\u30b9\u304c\u751f\u3058\u6613\u3044\u305f\u3081\u306b\u3001\u7a81\u7136\u5909\u7570\u7387\u304c\u9ad8\u3044\u3001\u307e\u305f\u3001PCR\u53ca\u3073\u96fb\u6c17\u6cf3\u52d5\u6cd5\u3092\u7528\u3044\u3066\u3001\u4e8c\u500d\u4f53\u751f\u7269\u3067\u306f\u4e21\u65b9\u306e\u89aa\u7531\u6765\u306e\u907a\u4f1d\u5b50\u578b\u3092\u5224\u5225\u53ef\u80fd\uff08\u5171\u512a\u6027\uff09\u3001\u6975\u5fae\u91cf\u306eDNA\u3067\u691c\u51fa\u53ef\u80fd\u3001\u3068\u3044\u3063\u305f\u7279\u5fb4\u306b\u3088\u308a\u3001\u91ce\u751f\u500b\u4f53\u7fa4\u306e\u96c6\u56e3\u907a\u4f1d\u5b66\u7684\u89e3\u6790\u304b\u3089\u3001\u89aa\u5b50\u9451\u5b9a\u3001\u72af\u7f6a\u635c\u67fb\u3068\u3044\u3063\u305f\u500b\u4f53\u9593\u306e\u95a2\u4fc2\u3092\u8abf\u67fb\u53ef\u80fd\u306a\u975e\u5e38\u306b\u6709\u7528\u306a\u907a\u4f1d\u30de\u30fc\u30ab\u30fc\u306b\u306a\u308a\u5f97\u308b\u3002\u305f\u3060\u3057\u3001\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u9818\u57df\u3092\u5bfe\u8c61\u751f\u7269\u7a2e\u306e\u30b2\u30ce\u30e0\u304b\u3089\u5358\u96e2\u3057\u3001\u305d\u306e\u4e21\u7aef\u306bPCR\u306e\u305f\u3081\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u5f93\u6765\u306e\u65b9\u6cd5\u3067\u306f\u3001\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3001\u30b5\u30f3\u30ac\u30fc\u6cd5\u306b\u3088\u308b\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3068\u3044\u3063\u305f\u6642\u9593\u53ca\u3073\u8cbb\u7528\u306e\u639b\u304b\u308b\u65b9\u6cd5\u3092\u53d6\u3089\u3056\u308b\u3092\u5f97\u305a\u3001\u30b2\u30ce\u30e0\u60c5\u5831\u306e\u306a\u3044\u91ce\u751f\u751f\u7269\u306b\u304a\u3044\u3066\u306f\u3001\u5c0e\u5165\u306b\u56f0\u96e3\u304c\u4f34\u3063\u305f\u3002<\/p>\n\n\n\n<p>\u3000\u305d\u306e\u3088\u3046\u306a\u4e2d\u30012010\u5e74\u9803\u306b\u767b\u5834\u3057\u305f\u6b21\u4e16\u4ee3\uff08\u8d85\u4e26\u5217\uff09\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u5229\u7528\u3059\u308c\u3070\u3001\u83ab\u5927\u306a\u6570\u306e\u30b2\u30ce\u30e0\u65ad\u7247\u306e\u60c5\u5831\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u305d\u306e\u4e00\u90e8\u306b\u542b\u307e\u308c\u308b\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u9818\u57df\u304b\u3089\u30de\u30fc\u30ab\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u3063\u3066\u304d\u305f\u3002\u7279\u306b\u3001\u6bd4\u8f03\u7684\u6700\u8fd1\u767b\u5834\u3057\u305f\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u306e\u4e2d\u3067\u3082 Ion PGM\u306f\u3001\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306e\u305f\u3081\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u4f5c\u6210\u306b\u5fc5\u8981\u306a\u6642\u9593\u53ca\u3073\u30b3\u30b9\u30c8\u304c\u7bc0\u7d04\u3067\u304d\u308b\u70b9\u3067\u512a\u308c\u305f\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u3067\u3042\u308b\u3002\u305d\u3053\u3067\u3001\u672c\u7814\u7a76\u306b\u304a\u3044\u3066\u306f\u3001\u307e\u3060\u5b9f\u7528\u4f8b\u306e\u5c11\u306a\u304b\u3063\u305fIon PGM\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u3092\u5229\u7528\u3057\u3001\u65b0\u898f\u65b9\u6cd5\u306b\u3088\u308b\u8fc5\u901f\u3067\u5b89\u4fa1\u306a\u958b\u767a\u3092\u8a66\u307f\u305f\u3002<\/p>\n\n\n\n<p>\u3000Ion PGM\u306f\u7d041Gbp\u3082\u306e\u30c7\u30fc\u30bf\u3092\u4e00\u5ea6\u306b\u51fa\u529b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u30013\u7a2e\u306e\u30ab\u30a8\u30eb\u985e\u306e\u30b2\u30ce\u30e0\u3092\u6df7\u5408\u3057\u3001\u30b2\u30ce\u30e0\u65ad\u7247\u306e\u60c5\u5831\u3092\u7db2\u7f85\u7684\u306b\u5f97\u305f\u3002\u305d\u306e\u5f8c\u3001\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u9818\u57df\u3092\u9078\u629c\u3057\u3066\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u30d7\u30ed\u30b0\u30e9\u30e0\u3092\u5229\u7528\u3057\u3066\u3001\u7db2\u7f85\u7684\u306b\u30de\u30fc\u30ab\u30fc\u3092\u8a2d\u8a08\u3057\u3001\u591a\u578b\u6027\u3092\u78ba\u8a8d\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u30013\u7a2e\u306e\u30ab\u30a8\u30eb\u985e\u306b\u304a\u3044\u3066\u5408\u8a08\u306761\u907a\u4f1d\u5b50\u5ea7\u306e\u30de\u30fc\u30ab\u30fc\u3092\u4e00\u5ea6\u306b\u8a2d\u8a08\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\u3002\u307e\u305f\u30011\u7a2e\u3042\u305f\u308a\u306b\u5fc5\u8981\u306a\u8cbb\u7528\u306f\u591a\u578b\u6027\u7b49\u306e\u78ba\u8a8d\u3082\u542b\u3081\u3066\u308215\u4e07\u5186\u7a0b\u5ea6\u3067\u3001\u671f\u9593\u30821\u30f6\u6708\u4ee5\u5185\u306b\u958b\u767a\u53ef\u80fd\u3067\u3042\u308a\u3001\u5f93\u6765\u306e\u5225\u306e\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u3092\u7528\u3044\u305f\u65b9\u6cd5\u3088\u308a\u3082\u6709\u7528\u3067\u3042\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002\u3055\u3089\u306b\u3001\u3053\u306e\u65b9\u6cd5\u3092\u7528\u3044\u3066\u3001\u897f\u5357\u8af8\u5cf6\u7523\u7d76\u6ec5\u5371\u60e7\u4e21\u751f\u985e\u3067\u3042\u308b\u30cf\u30ca\u30b5\u30ad\u30ac\u30a8\u30eb\u7a2e\u7fa4\uff08<em>Odorrana narina, O. amamiensis, O. supranarina, O. utsunomiyaorum<\/em>\uff09\u306b\u304a\u3044\u3066\u5b9f\u969b\u306b\u30de\u30fc\u30ab\u30fc\u958b\u767a\u3092\u884c\u3063\u305f\u3068\u3053\u308d\u3001\u8907\u6570\u7a2e\u306b\u304a\u3044\u3066\u6709\u52b9\u3067\u3001\u5341\u5206\u306a\u591a\u578b\u6027\u3092\u793a\u305920\u5ea7\u306e\u7406\u60f3\u7684\u306a\u30de\u30fc\u30ab\u30fc\u304c\u5358\u96e2\u3067\u304d\u305f\u3002\u672c\u6cd5\u306f\u4ed6\u306e\u91ce\u751f\u751f\u7269\u306b\u304a\u3044\u3066\u3082\u9069\u5fdc\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304b\u3089\u3001\u5f53\u8a72\u5206\u91ce\u306b\u304a\u3051\u308b\u6ce8\u76ee\u5ea6\u306f\u9ad8\u3044\u3002\u5b9f\u969b\u306b\u672c\u7814\u7a76\u8ad6\u6587\u3092\u898b\u305f\u56fd\u5185\u5916\u306e\u7814\u7a76\u8005\u304b\u3089\u306e\u5354\u529b\u306e\u8981\u8acb\u304c\u3042\u308a\u3001\u3059\u3067\u306b\u5171\u540c\u7814\u7a76\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u308b\u3002\u307e\u305f\u672c\u6cd5\u306b\u3088\u3063\u3066\u3001\u5206\u5b50\u30de\u30fc\u30ab\u30fc\u306e\u5229\u7528\u304c\u5bb9\u6613\u306b\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u3001\u5c06\u6765\u7684\u306b\u306f\u69d8\u3005\u306a\u5206\u91ce\u306e\u7814\u7a76\u8005\u304c\u7c21\u5358\u306b\u907a\u4f1d\u5b50\u9451\u5b9a\u7b49\u3092\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u306b\u306a\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p>\n\n\n\n<p>\u3000\u672c\u7814\u7a76\u306f\u3001\u4e3b\u306b\u56fd\u7acb\u907a\u4f1d\u5b66\u7814\u7a76\u6240\u3001\u91ce\u6fa4\u660c\u6587\u535a\u58eb\u3068\u306e\u5171\u540c\u7814\u7a76\u306b\u3088\u308a\u884c\u308f\u308c\u305f\u3002\u307e\u305f\u3001\u7814\u7a76\u6210\u679c\u306f\u3001\u5f53\u8a72\u5206\u91ce\u306e\u56fd\u969b\u8a8c\u3067\u3042\u308b<em>Journal of Heredity<\/em>\u8a8c\uff085-year I.F. = 2.417\uff09\u306b\u6295\u7a3f\u3057\u3001\u63b2\u8f09\u3055\u308c\u305f\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u672c\u7814\u7a76\u3067\u306f\u3001\u96c6\u56e3\u907a\u4f1d\u5b66\u7684\u89e3\u6790\u306b\u304a\u3044\u3066\u6709<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"colormag_page_container_layout":"default_layout","colormag_page_sidebar_layout":"default_layout","_locale":"ja","_original_post":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=1627","footnotes":""},"categories":[20],"tags":[],"class_list":["post-1627","post","type-post","status-publish","format-standard","hentry","category-20","ja"],"_links":{"self":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1627","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1627"}],"version-history":[{"count":3,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1627\/revisions"}],"predecessor-version":[{"id":1630,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1627\/revisions\/1630"}],"wp:attachment":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1627"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1627"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1627"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}