{"id":2689,"date":"2024-04-25T11:21:03","date_gmt":"2024-04-25T02:21:03","guid":{"rendered":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=2689"},"modified":"2024-04-25T16:10:22","modified_gmt":"2024-04-25T07:10:22","slug":"%e3%83%84%e3%83%a1%e3%82%ac%e3%82%a8%e3%83%ab%e8%83%9a%e3%82%92%e4%bd%bf%e3%81%a3%e3%81%9f%e6%96%b0%e3%81%97%e3%81%84%e3%83%88%e3%83%a9%e3%83%b3%e3%82%b9%e3%82%b8%e3%82%a7%e3%83%8b%e3%83%83%e3%82%af","status":"publish","type":"post","link":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=2689","title":{"rendered":"\u30c4\u30e1\u30ac\u30a8\u30eb\u80da\u3092\u4f7f\u3063\u305f\u65b0\u3057\u3044\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30ec\u30dd\u30fc\u30bf\u30fc\u5b9f\u9a13\u7cfb\u3068\u4e09\u6b21\u5143\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u89e3\u6790\u7cfb\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002"},"content":{"rendered":"\n<p>\u9032\u5316\u767a\u751f\u30b2\u30ce\u30df\u30af\u30b9\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306e\u5927\u5b66\u9662\u751f\u306e\u5742\u53e3\u88d5\u4ecb\u3055\u3093\u3068\u4e95\u5ddd\u304c\u7b46\u982d\u8457\u8005\u306e\u8ad6\u6587\u304cDevelopment Growth &amp; Differentiation\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002\u30c4\u30e1\u30ac\u30a8\u30eb\u306f\u767a\u751f\u7814\u7a76\u306b\u91cd\u8981\u306a\u30e2\u30c7\u30eb\u52d5\u7269\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u305d\u306e\u80da\u306f\u5375\u9ec4\u3092\u591a\u91cf\u306b\u542b\u307f\u4e0d\u900f\u660e\u306a\u305f\u3081\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u7b49\u3092\u7528\u3044\u305f3\u6b21\u5143\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u89e3\u6790\u306f\u3001\u80da\u306e\u8868\u9762\u69cb\u9020\u3084\u57f9\u990a\u7d44\u7e54\u7247\u3001\u80da\u767a\u751f\u304c\u7d42\u4e86\u3057\u305f\u5f8c\u306e\u5e7c\u751f\uff08\u30aa\u30bf\u30de\u30b8\u30e3\u30af\u30b7\uff09\u306b\u9650\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u672c\u7814\u7a76\u3067\u306f\u3053\u306e\u554f\u984c\u3092\u89e3\u6c7a\u3059\u308b\u305f\u3081\u3001\u307e\u305a\u71b1\u8010\u6027\u3092\u6301\u3064\u30d2\u30c8\u80ce\u76e4\u578b\u30a2\u30eb\u30ab\u30ea\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\uff08PLAP\uff09\u3092\u7d44\u7e54\u7279\u7570\u7684\u306a\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u7528\u3044\u3066\u76ee\u7684\u7d44\u7e54\u3067\u767a\u73fe\u3055\u305b\u3001\u71b1\u51e6\u7406\u306b\u3088\u308a\u5185\u5728\u306e\u30a2\u30eb\u30ab\u30ea\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3092\u5931\u6d3b\u3055\u305b\u305f\u5f8c\u3001\u30a2\u30eb\u30ab\u30ea\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u57fa\u8cea\u3092\u53cd\u5fdc\u3055\u305b\u3066\u76ee\u7684\u7d44\u7e54\u3092\u67d3\u8272\u3059\u308b\u65b9\u6cd5\u3092\u78ba\u7acb\u3057\u307e\u3057\u305f\u3002\u6b21\u306b\u3053\u306e\u65b9\u6cd5\u3067\u51e6\u7406\u3057\u305f\u80da\u306e\u9023\u7d9a\u5207\u7247\u3092\u81ea\u52d5\u64ae\u5f71\u3057\u3001\u30b3\u30f3\u30d4\u30e5\u30fc\u30bf\u30fc\u4e0a\u3067\u6a19\u7684\u7d44\u7e54\u306e\u7acb\u4f53\u50cf\u3092\uff13\u6b21\u5143\u69cb\u7bc9\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f\uff08Correlative Light Microscopy and Block-face Imaging (CoMBI) method\uff09\u3002\u4ee5\u4e0a\u306e\u300cPLAP-CoMBI\u6cd5\u300d\u306f\u3001\u30c4\u30e1\u30ac\u30a8\u30eb\u80da\u306b\u304a\u3044\u3066\u3001\u5f93\u6765\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u308b\u65b9\u6cd5\u3067\u306f\u6210\u3057\u5f97\u306a\u304b\u3063\u305f3D\u30c7\u30b8\u30bf\u30eb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3092\u53ef\u80fd\u306b\u3059\u308b\u3082\u306e\u3067\u3059\u3002<\/p>\n\n\n\n<p>\uff08\u8ad6\u6587\u306e\u88cf\u8a71\uff09<br>\u3053\u306e\u7814\u7a76\u306e\u304d\u3063\u304b\u3051\u306f\u79c1\u3068\u837b\u91ce\u3055\u3093\u304c\u7740\u4efb\u76f4\u5f8c\u306b\u51fa\u5e2d\u3057\u305f\u7814\u7a76\u4f1a\uff08<a href=\"https:\/\/www.nibb.ac.jp\/newmodel\/meeting\/20170824-25.pdf\">\u7b2c3\u56de\u6b21\u4e16\u4ee3\u4e21\u751f\u985e\u7814\u7a76\u4f1a<\/a>\u30002017\u5e748\u670824 &#8211; 25\u65e5\u958b\u50ac\uff09\u3067CoMBI\u3092\u4f7f\u3063\u305f\u7814\u7a76 (<a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/dgd.12827\">Sutrisno et al. 2022<\/a>) \u3092\u76ee\u306b\u3057\u305f\u3053\u3068\u3067\u3057\u305f\u3002\u79c1\u306f\u8840\u7ba1\u307e\u3067\u304d\u308c\u3044\u306b\u518d\u69cb\u7bc9\u3055\u308c\u305f\u4e09\u6b21\u5143\u753b\u50cf\u306b\u305f\u3060\u305f\u3060\u5727\u5012\u3055\u308c\u307e\u3057\u305f\u304c\u3001\u524d\u4efb\u6821\u3067PLAP\u3092\u4f7f\u3063\u305f\u30ec\u30dd\u30fc\u30bf\u30fc\u7cfb\u3092\u958b\u767a\u3057\u3066\u3044\u305f\u837b\u91ce\u3055\u3093\u306f\u77ac\u6642\u306b\u3001\u65b0\u3057\u3044\u3053\u3068\u304c\u3067\u304d\u308b\u3068\u601d\u3044\u3064\u3044\u305f\u3088\u3046\u3067\u3057\u305f\u3002\u307e\u305f\u3001\u5076\u7136\u306b\u3082\u79c1\u304c\u8da3\u5473\u3068\u5b9f\u76ca\uff08\u6c34\u69fd\u306e\u6e29\u5ea6\u76e3\u8996\u306a\u3069\uff09\u3092\u517c\u306d\u3066\u30de\u30a4\u30b3\u30f3\u306e\u30d7\u30ed\u30b0\u30e9\u30df\u30f3\u30b0\u3092\u59cb\u3081\u3066\u3044\u305f\u306e\u3067\u3001CoMBI\u3067\u7528\u3044\u308b\u81ea\u52d5\u64ae\u5f71\u88c5\u7f6e\u306e\u30b7\u30b9\u30c6\u30e0\u306f\u3059\u3050\u306b\u7406\u89e3\u3067\u304d\u307e\u3057\u305f\u3002\u305d\u306e\u305f\u3081\u3001\u5e83\u5cf6\u306b\u623b\u3063\u3066\u3059\u3050\u306bCoMBI\u306e\u539f\u8457\u8ad6\u6587 (<a href=\"https:\/\/www.nature.com\/articles\/s41598-017-03900-9\">Tajika et al., 2017<\/a>) \u3092\u8aad\u3093\u3067\u72ec\u529b\u3067\u4f5c\u308a\u59cb\u3081\u3001\u4e09\u304b\u6708\u5f8c\u306e11\u6708\u5f8c\u534a\u306b\u306f\u5b8c\u6210\u3055\u305b\u3066\u3044\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001\u52d5\u7269\u5b66\u4f1a\u3067CoMBI\u958b\u767a\u8005\u306e\u591a\u9e7f\u3055\u3093\u3068\u306e\u5076\u767a\u7684\u306a\u51fa\u4f1a\u3044\u3068\u4ea4\u6d41\u3092\u306f\u3055\u307f\u3064\u3064\u3001\u6642\u9593\u306f\u304b\u304b\u308a\u307e\u3057\u305f\u304c\u837b\u91ce\u5148\u751f\u304c\u4e2d\u5fc3\u3068\u306a\u3063\u3066\u30c7\u30fc\u30bf\u3092\u307e\u3068\u3081\u3042\u3052\u3066\u4eca\u56de\u306e\u8ad6\u6587\u767a\u8868\u306b\u81f3\u308a\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<p>Sakagami, K., Igawa, T., Saikawa, K., Sakaguchi, Y., Hossain, N., Kato, C., Kinemori, K., Suzuki, N., Suzuki, M., Kawaguchi, A., Ochi, H., Tajika, Y., &amp; Ogino, H. (2024).\u3000Development of a heat-stable alkaline phosphatase reporter system for cis-regulatory analysis and its application to 3D digital imaging of Xenopus embryonic tissues. <em>Development, growth &amp; differentiation<\/em>, 66: 256-265. 10.1111\/dgd.12919.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"eager\" decoding=\"async\" width=\"780\" height=\"520\" src=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/04\/sakagami2024.png\" alt=\"\" class=\"wp-image-2690\" style=\"width:807px;height:auto\" srcset=\"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/04\/sakagami2024.png 780w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/04\/sakagami2024-300x200.png 300w, https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/wp-content\/uploads\/2024\/04\/sakagami2024-768x512.png 768w\" sizes=\"(max-width: 780px) 100vw, 780px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u9032\u5316\u767a\u751f\u30b2\u30ce\u30df\u30af\u30b9\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306e\u5927\u5b66\u9662\u751f<\/p>\n","protected":false},"author":1,"featured_media":2690,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"colormag_page_container_layout":"default_layout","colormag_page_sidebar_layout":"default_layout","_locale":"ja","_original_post":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=2689","footnotes":""},"categories":[6],"tags":[],"class_list":["post-2689","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-topics","ja"],"_links":{"self":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/2689","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2689"}],"version-history":[{"count":4,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/2689\/revisions"}],"predecessor-version":[{"id":2697,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/2689\/revisions\/2697"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/media\/2690"}],"wp:attachment":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2689"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2689"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2689"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}