{"id":293,"date":"2017-09-01T10:28:52","date_gmt":"2017-09-01T01:28:52","guid":{"rendered":"http:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=293"},"modified":"2019-10-17T22:15:26","modified_gmt":"2019-10-17T13:15:26","slug":"%e3%83%9f%e3%83%89%e3%83%ab%e3%82%b9%e3%83%ab%e3%83%bc%e3%83%97%e3%83%83%e3%83%88%e3%83%bb%e3%82%b5%e3%83%b3%e3%82%ac%e3%83%bc%e3%82%b7%e3%83%bc%e3%82%af%e3%82%a8%e3%83%b3%e3%82%b7%e3%83%b3%e3%82%b0","status":"publish","type":"post","link":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/?p=293","title":{"rendered":"[atbench]\u30df\u30c9\u30eb\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u30fb\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0"},"content":{"rendered":"

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\n\u3053\u306e\u6a5f\u7a2e\u306f\u672c\u683c\u7684\u306a\u30d7\u30ec\u30fc\u30c8\u9060\u5fc3\u6a5f\u306b\u6bd4\u3079\u308b\u3068\u5c11\u3057\u901f\u5ea6\u304c\u8db3\u308a\u306a\u3044\u3088\u3046\u306b\u601d\u3048\u307e\u3059\u304c\u3001\u554f\u984c\u306a\u304f\u30a8\u30bf\u6c88\u304c\u3067\u304d\u307e\u3059\u3002\u6700\u5927G\u304cABI\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u7cbe\u88fd\u306e\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\uff08\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\uff09\u306e\u4e0b\u9650\u5024\uff081.4k\u00d7G\uff09\u306a\u306e\u304c\u30df\u30bd\u3067\u3059\u3002\u79c1\u306fKUBOTA\u793e\u304cABI\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u898b\u3066\u88fd\u54c1\u306e\u4ed5\u69d8\u3092\u6c7a\u3081\u305f\u306e\u304b\u3068\u601d\u3063\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u5b9f\u969b\u306b\u306f\u5076\u7136\u3089\u3057\u3044\u3067\u3059\uff08\u4e45\u4fdd\u7530\u3055\u3093\u304c\u53d6\u6750\u306b\u6765\u3089\u308c\u3066\u77e5\u308a\u307e\u3057\u305f\u3002\uff09\u3002\u79c1\u306b\u3068\u3063\u3066\u306f\u81ea\u5206\u306e\u7814\u7a76\u8cbb\u3067\u8cb7\u3048\u308b\u3053\u306e\u88fd\u54c1\u306e\u4fa1\u683c\u3068\u4ed5\u69d8\u304c\u7d76\u5999\u3060\u3063\u305f\u306e\u3067\u3001\u305d\u306e\u5f8c\u306e\u5b9f\u9a13\u304c\u304b\u306a\u308a\u6357\u308a\u307e\u3057\u305f\u3002\u79c1\u306e\u8a71\u3092\u805e\u3044\u3066\u3044\u305f\u3060\u3044\u305f\u5f8c\u306b\u3001\u88fd\u54c1\u306e\u30b9\u30da\u30c3\u30af\u306f\u73fe\u5728\u306f\u5f37\u5316\u3055\u308c\u3066MAX 1.53K\u00d7G\u3068\u306a\u308a\u3001\u4eca\u306f\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u4e0b\u9650\u5024\u304b\u3089\u3082\u3046\u5c11\u3057\u4f59\u88d5\u304c\u3042\u308a\u307e\u3059\u3002<\/span><\/p>\n

\u4ee5\u4e0b\u306f\u307b\u3068\u3093\u3069\u3001ABI\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3068\u540c\u3058\u3067\u3059\u304c\u3001\u4e00\u5fdc\u3001\u7d30\u304b\u3044\u90e8\u5206\u3092\u542b\u3081\u3066\u8a18\u8ff0\u3057\u3066\u304a\u304d\u307e\u3059\u3002<\/span><\/p>\n

    \n
  1. \u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\uff08\u4ed6\u30b5\u30a4\u30c8\u306b\u3082\u3042\u308b\u7bc0\u7d04\u6cd5\uff09\u306f\u300196well\u30d7\u30ec\u30fc\u30c8\u3067\u884c\u3046\u3002\u4f38\u9577\u53cd\u5fdc\u306e\u6642\u9593\u306f4\u5206\u3082\u3044\u3089\u306a\u3044\u30022\u5206\u3067OK\u3002<\/span><\/li>\n
  2. \u53cd\u5fdc\u6e08\u307f\u30b5\u30f3\u30d7\u30eb10\u03bcL\u306b\u3001125mM EDTA 2.5\u03bcL\u3001100%\u30a8\u30bf\u30ce\u30fc\u30eb30\u03bcL\u3092\u6dfb\u52a0\u3059\u308b\u3002<\/span>
    \n\u4e01\u5be7\u306b\u884c\u3046\u5fc5\u8981\u306f\u306a\u304f\u3001well\u306e\u58c1\u306b\u4ed8\u3051\u308b\u304b\u3001\u4e0a\u304b\u3089\u6ef4\u4e0b\u3059\u308b\u3060\u3051\u3067OK\u3002\u5206\u6ce8\u30d4\u30da\u30c3\u30c8\u30de\u30f3\u3001\u96fb\u52d5\u30d4\u30da\u30c3\u30c8\u30de\u30f3\u3092\u99c6\u4f7f\u3059\u308b\u3068\u4fbf\u5229\u3002<\/span><\/li>\n
  3. \u30b5\u30f3\u30d7\u30eb\u306e\u5165\u3063\u305fwell\u306e\u90e8\u5206\u306b\u30a2\u30eb\u30df\u30b7\u30fc\u30eb\u3092\u5f35\u308a\u3064\u3051\u30014\u56de\u8ee2\u5012\u6df7\u548c\u3002\u3053\u306e\u3068\u304d\u3001\u6db2\u304c\u52d5\u304b\u306a\u3044\u5834\u5408\u304c\u3042\u308b\u306e\u3067\u3001\u9006\u3055\u306b\u3057\u305f\u3068\u304d\u306b\u5c11\u3057\u30bf\u30c3\u30d4\u30f3\u30b0\u3057\u3066\u3084\u308b\u3068\u826f\u3044\u3002<\/span><\/li>\n
  4. 15\u5206\u9593\u3001\u5ba4\u6e29\u653e\u7f6e<\/span><\/li>\n
  5. \u30d7\u30ec\u30fc\u30c8\u5c02\u7528\u9060\u5fc3\u6a5f PlateSpin\u00ae\u2161<\/a>\u306b\u30bb\u30c3\u30c8\u3057\u3001\u6700\u5927\u901f\u5ea6\u306745\u5206\u9593\u9060\u5fc3\u3059\u308b\u3002<\/span><\/li>\n
  6. \u30d7\u30ec\u30fc\u30c8\u3092\u88cf\u8fd4\u3057\u3066\u3001\u5c11\u3057\u3060\u3051\u9060\u5fc3\u3059\u308b\uff08100\u00d7G\u7a0b\u5ea6\uff09\u3002\u5f53\u7136\u306a\u304c\u3089\u6db2\u304c\u51fa\u3066\u304f\u308b\u306e\u3067\u3001\u53d7\u3051\u76bf\u7684\u306a\u3082\u306e\uff08\u30c1\u30c3\u30d7\u30b1\u30fc\u30b9\u306a\u3069\uff09\u3092\u7528\u610f\u3059\u308b\u304b\u3001\u30ad\u30e0\u30bf\u30aa\u30eb\u306a\u3069\u3092\u3042\u3066\u304c\u3063\u3066\u304a\u304f\u3002<\/span><\/li>\n
  7. 70%\u30a8\u30bf\u30ce\u30fc\u30eb60\u03bcL\u3092\u6dfb\u52a0\u3059\u308b\uff08\u3053\u306e\u3068\u304d\u3082\u5206\u6ce8\u30d4\u30da\u30c3\u30c8\u30de\u30f3\u3042\u308b\u3068\u4fbf\u5229\uff09\u3002<\/span><\/li>\n
  8. \u518d\u3073\u30d7\u30ec\u30fc\u30c8\u5c02\u7528\u9060\u5fc3\u6a5f PlateSpin\u00ae\u2161<\/a>\u306b\u30bb\u30c3\u30c8\u3057\u3001\u6700\u5927\u901f\u5ea6\u306715\u5206\u9593\u9060\u5fc3\u3059\u308b\u3002<\/span><\/li>\n
  9. \u518d\u3073\u88cf\u8fd4\u3057\u3066\u3001\u5c11\u3057\u3060\u3051\u9060\u5fc3\u3059\u308b\uff08100\u00d7G\u7a0b\u5ea6\uff09\u3002\u3053\u306e\u3068\u304d\u3082\u5ec3\u6db2\u53d7\u3051\u3092\u30bb\u30c3\u30c8\u3057\u3066\u304a\u304f\uff08\u305d\u3046\u3057\u306a\u3044\u3068\u9060\u5fc3\u6a5f\u306e\u4e2d\u306b70%\u30a8\u30bf\u30ce\u30fc\u30eb\u304c\u98db\u6563\u3057\u3001\u653e\u7f6e\u3059\u308b\u3068\u6700\u7d42\u7684\u306b\u9306\u3073\u308b\uff09\u3002<\/span><\/li>\n
  10. \u305d\u306e\u307e\u307e\u5ba4\u6e29\u3067\u653e\u7f6e\u3057\u3001\u4e7e\u71e5\u3055\u305b\u308b\u3002\u30b4\u30df\u306a\u3069\u304c\u5165\u308b\u306e\u3092\u9632\u3050\u305f\u3081\u30ad\u30e0\u30ef\u30a4\u30d7\u306a\u3069\u3092\u8f09\u305b\u3066\u304a\u304f\u3002<\/span><\/li>\n
  11. Hidi\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c912\u03bcL\u3092\u6dfb\u52a0\u3057\u3001\u30a2\u30eb\u30df\u30b7\u30fc\u30eb\u3092\u5f35\u308a\u3064\u3051\u3066\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3002\u4e00\u5ea6\u30b9\u30d4\u30f3\u30c0\u30a6\u30f3\u3057\u3001\u30bb\u30d7\u30bf\u3092\u4ed8\u3051\u3066\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc\u306b\u30bb\u30c3\u30c8\uff08\u30d2\u30fc\u30c8\u30b7\u30e7\u30c3\u30af\u306f\u5fc5\u8981\u306a\u3057\uff09\u3002<\/span><\/li>\n<\/ol>\n

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