Although environmental DNA has now become a standard tool in ecological research, it requires expensive equipment and reagents such as real-time PCR instruments and fluorescent probes, and the laboratories that can prepare them are limited.<\/p>\n
Therefore, we have developed a method for efficiently detecting multiple species simultaneously and inexpensively from environmental water by applying the PCR-RFLP (PCR product restriction enzyme fragment length polymorphism) method. This method does not require a real-time PCR device or fluorescently labeled oligo DNA, and can be used for experiments only with a PCR device and an electrophoresis device in a general laboratory. It can be implemented even in laboratories mainly for ecological research, and some junior high and high schools with PCR devices.<\/p>\n
The target species, the Japanese brown frog (Rana<\/em> japonica<\/em>), the mountain brown frog (Rana ornativentris<\/em>), and the Tago frog (Rana tagoi tagoi<\/em>), are common amphibians that are often found in rural and mountain villages of Japan and are also key indicators for environmental monitoring. I hope that the method developed this time will help build a sustainable society in harmony with nature.<\/p>\n Reference\uff1a*Igawa T, *Takahara T, Lau Q, Komaki S, An application of PCR-RFLP species identification assay for environmental DNA detection. PeerJ<\/em> 7: e7597 October 2019. [\u00a0DOI<\/a>\u00a0|\u00a0http<\/a>\u00a0|\u00a0PDF<\/a>\u00a0] Although environment<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"colormag_page_container_layout":"default_layout","colormag_page_sidebar_layout":"default_layout","_locale":"en_US","_original_post":"991","footnotes":""},"categories":[6],"tags":[],"class_list":["post-1031","post","type-post","status-publish","format-standard","hentry","category-topics","en-US"],"_links":{"self":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1031","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1031"}],"version-history":[{"count":2,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1031\/revisions"}],"predecessor-version":[{"id":1098,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=\/wp\/v2\/posts\/1031\/revisions\/1098"}],"wp:attachment":[{"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1031"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1031"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/home.hiroshima-u.ac.jp\/~tigawa\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1031"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n*co-corresponding authors<\/p>\n","protected":false},"excerpt":{"rendered":"