Using mouse embryonic stem (ES) cells as a model, we aim to elucidate the regulatory mechanism of a phenomenon called transcriptional bursting using a novel experimental approach that combines genome editing technology, live imaging, and cell biology. We are also working on elucidating the molecular mechanism of cell-to-cell heterogeneity in gene expression levels in mouse ES cells. We are also working on the development of an efficient construction method for ZFNs, a genome editing tool.
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- New preprint about “STREAMING-tag” from the lab now out in bioRxiv!Our “STREAMING-tag” paper is now available on bioRxiv!https://bit.ly/3JODuoX In this study, we revealed spatiotemporal relationships between regulatory factors and transcriptional activity! Transcription dynamically switches between ON and OFF states, but the regulatory mechanism is not fully understood. Mediator, BRD4, and non-phosphorylated RNA polymerase II (RNAPII) has been shown to form clusters around gene regions in the ON state. In the ON state, gene loci can be visualized by the MS2 system, but this is not the case in the OFF state. To understand the regulatory mechanism of transcriptional bursting, it is necessary to understand what is happening not only in the ON state but also in the OFF state. Therefore, we established the “STREAMING-tag” system, which can quantify gene transcriptional activity and subcellular localization of genes near the TSS with minimal effect on gene function. See the paper for details! https://bit.ly/3JODuoX We applied this system to Nanog in mouse ES […]
- Ochiai organized WS “[1PW-04] Chromatin Potential from Single Cell Analysis” at MBSJ2020The 43rd Annual Meeting of the Molecular Biology Society of Japan was held online December 2-4, 2021, and I organized and chaired the workshop “[1PW-04] Chromatin potential revealed by single cell analysis” with Dr. Ichiro Hiratani (RIKEN).I also gave a presentation titled “Regulation of transcriptional bursting by chromatin potential”.
- Our transcriptional bursting paper is finally out at Science Advances!Happy to have our work on the kinetic properties of transcriptional bursting comprehensively analyzed in mouse embryonic stem cells finally published in Science Advances.https://advances.sciencemag.org/content/6/25/eaaz6699