- 2022年1月8日
- 2022年1月8日
New preprint about “STREAMING-tag” from the lab now out in bioRxiv!
Our “STREAMING-tag” paper is now available on bioRxiv!https://bit.ly/3JODuoX In this study, we revealed spatiotemporal relationships between regulatory factors and transcriptional activity! Transcription dynamically switches between ON and OFF states, but the regulatory mechanism is not fully understood. Mediator, BRD4, and non-phosphorylated RNA polymerase II (RNAPII) has been shown to form clusters around gene regions in the ON state. In the ON state, gene loci can be visualized by the MS2 system, but this is not the case in the OFF state. To understand the regulatory mechanism of transcriptional bursting, it is necessary to understand what is happening not only in the ON state but also in the OFF state. Therefore, we established the “STREAMING-tag” system, which can quantify gene transcriptional activity and subcellular localization of genes near the TSS with minimal effect on gene function. See the paper for details! https://bit.ly/3JODuoX We applied this system to Nanog in mouse ES […]