Our laboratory has moved to the Division of Gene Expression Dynamics, Medical Research Center Initiative for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, as of March 1, 2023.New HP : https://www.bioreg.kyushu-u.ac.jp/labo/ged/en/
A paper on the “STREAMING-Tag” system, a joint research project led by researcher Ohishi-san and the groups of Prof. Hiroshi Kimura at the Tokyo Institute of Technology and Prof. Yasuyuki Ohkawa at the Medical Institute of Bioregulation, Kyushu University, has been published in Nature Communications.https://www.nature.com/articles/s41467-022-35286-2 In this paper, we established the Spliced TetO REpeAt, MS2 repeat, and INtein sandwiched reporter Gene tag (STREAMING-tag) system that enables highly precise quantification of transcriptional activity and nuclear localization of specific endogenous genes. Using this system and fluorescent protein knock-in to transcription-related factors, we have revealed that specific transcription-related factors form clusters around genes in a transcriptional activity state-dependent manner. This system is expected to contribute to the elucidation of detailed transcriptional regulation mechanisms.
Ochiai’s review article titled “Facilitating genome function understanding using genome editing dependent bioimaging techniques” was published in Gene and Genome Editing. https://www.sciencedirect.com/science/article/pii/S2666388022000120?via%3Dihub
https://link.springer.com/protocol/10.1007/978-1-0716-2724-2_8
Ohishi-san, a research scientist, received the Outstanding Researcher Award at the 7th Annual Meeting of the Japanese Society for Genome Editing.
Our “STREAMING-tag” paper is now available on bioRxiv!https://bit.ly/3JODuoX In this study, we revealed spatiotemporal relationships between regulatory factors and transcriptional activity! Transcription dynamically switches between ON and OFF states, but the regulatory mechanism is not fully understood. Mediator, BRD4, and non-phosphorylated RNA polymerase II (RNAPII) has been shown to form clusters around gene regions in the ON state. In the ON state, gene loci can be visualized by the MS2 system, but this is not the case in the OFF state. To understand the regulatory mechanism of transcriptional bursting, it is necessary to understand what is happening not only in the ON state but also in the OFF state. Therefore, we established the “STREAMING-tag” system, which can quantify gene transcriptional activity and subcellular localization of genes near the TSS with minimal effect on gene function. See the paper for details! https://bit.ly/3JODuoX We applied this system to Nanog in mouse ES […]
The 43rd Annual Meeting of the Molecular Biology Society of Japan was held online December 2-4, 2021, and I organized and chaired the workshop “[1PW-04] Chromatin potential revealed by single cell analysis” with Dr. Ichiro Hiratani (RIKEN).I also gave a presentation titled “Regulation of transcriptional bursting by chromatin potential”.
Happy to have our work on the kinetic properties of transcriptional bursting comprehensively analyzed in mouse embryonic stem cells finally published in Science Advances.https://advances.sciencemag.org/content/6/25/eaaz6699