Graduate School of Integrated Sciences for Life
Program for Biotechnology
Hiroshima University

Kuroda Lab.

    

Direction

 
Our research group has focused on “biomolecules-material interface”, “phosphorus and biomolecules”, “silicon and biomolecules” and developed environmental biotechnology as well as biosensing that contributes to our health.

 

STUDY 01

Biomolecules-material interface

This group is studying on proteins/peptides that capture interfaces of inorganic/organic materials. For example, asbestos detection method has been developed using asbestos-binding peptides. The exosome-binding peptides are now used for isolation/analysis of exosomes. They are developing a new method to modify the interface of silicon devices.

STUDY 02

Phosphorus and biomolecules

There are metabolic pathways of phosphate polymer “polyphosphate” and of +3 oxidation state of phosphorus “phosphite”. ATP can be synthesized using polyphosphate and ADP. NADH can be generated from NAD and phosphite. This group is studying on application of these metabolic enzymes to bio-production. They utilize these genes to develop phosphorus accumulating bacteria as well as phosphite-dependent organisms for biocontainment.

 

 

 

STUDY 03

Silicon and biomolecules

Although silicon metabolism is well-known in diatoms and plants, this group is considering that it has evolved from microorganisms. They found that a group of Bacillus incorpotates silicon on their spore surfaces and are now studying on the origin of silicon metabolism of life.

 

Papers

 

Bio-material interface

T. Ishida, N. Fujihara, T. Nishimura, H. Funabashi, R. Hirota, T. Ikeda, and A. Kuroda
Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy
Genes and Environment, 41, 14-25 (2019) 
 
T. Ishida, M. Alexandrov, T. Nishimura, K. Minakawa, R. Hirota, K. Sekiguchi, N. Kohyama, and A. Kuroda
Molecular engineering of a fluorescent bioprobe for sensitive and selective detection of amphibole asbestos
PLos ONE, 8(9):e76231 (2013)
 
T. Ikeda, A. Kuroda
Why does the silica-binding protein "Si-tag" strongly bind to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces
Colloids Surf. B: Biointerfaces, 86(2), 359-363 (2011) 
 

Phosphorus and biomolecules

R. Hirota, K. Abe, Z. Katsuura, R. Noguchi, S. Moribe, K. Motomura, T. Ishida, M. Alexandrov, H. Funabashi, T. Ikeda & A. Kuroda
A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite
Scientific Reports, 7:44748 (2017)

 
K. Motomura, R. Hirota, M. Okada, T. Ikeda, T. Ishida, and A. Kuroda
A new subfamily of polyphosphate kinase 2 (class III PPK2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation, Appl. Environ. Microbiol. 80(8), 2602-2608 (2014)
 
S. Iwamoto, K. Motomura, Y. Shinoda, M. Urata, J. Kato, N. Takiguchi, H. Ohtake, R. Hirota, A. Kuroda
Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate
Appl. Environ. Microbiol. 73, 5676-5678 (2007)
 
T. Morohoshi, T. Maruo, Y. Shirai, J. Kato, T. Ikeda, N. Takiguchi, H. Ohtake, A. Kuroda
Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp.
Appl. Environ. Microbiol., 68, 4107-4110 (2002)
 

Silicon and biomolecules 

R. Hirota, Y. Hata, T. Ikeda, T. Ishida, A. Kuroda
The silicon layer supports acid resistance of Bacillus cereus spore
J. Bacteriol. 192, 111-116 (2010) 
 
K.Motomura, T. Ikeda, S. Matsuyama, M. A. A. Abdelhamid, T. Tanaka, T. Ishida, R. Hirota, A. Kuroda,
The C-terminal zwitterionic sequence of CotB1 Is essential for biosilicification of the Bacillus cereus spore coat, J. Bacteriol., 198,  276-282 (2016)
 

Biosensing (Fluorescence, Luminescence)

A. Kuroda, Alexandrov M., Nishimura T., Ishida T
Rapid on-site detection of airborne asbestos fibers and potentially hazardous nanomaterials using fluorescence microscopy-based biosensing
Biotechnol J. 11:757-767 (2016)
 
K. Noda, H. Goto, Y. Murakami, A.B.F. Ahmed, A. Kuroda
Endotoxin assay by bioluminescence using mutant firefly luciferase
Anal. Biochem. 397, 152-155 (2010) 
 
T. Satoh, J. Kato, N. Takiguchi, H. Ohtake, A. Kuroda
ATP amplification for ultrasensitive bioluminescence assay: detection of a single bacterial cell
Biosci. Biotechnol. Biochem. 68, 1216-1220 (2004)
  
 

Request or Contact information

mail to: akuroda(at)hiroshima-u.ac.jp
(at) should be replaced with @
 

Akio Kuroda's lab | Hiroshima University (HU) - ResearchGate

 

Other papers