Achivement

我々の実績

2020

T. Ishida, T. Hashimoto, K. Masaki, H. Funabashi, R. Hirota, T. Ikeda, H. Tajima, A. Kuroda,  
Application of peptides with an affinity for phospholipid membranes during the automated purification of extracellular vesicles,
Scientific Reports, vol. 10, Article number: 18718 (2020)
https://doi.org/10.1038/s41598-020-75561-0
 
S. Hino, K. Sugikawa, R. Kawasaki, H. Funabashi, A. Kuroda, A. Ikeda, 
Aggregation-induced emission and retention of crystal chiral information of tetraphenylethylene incorporated by polysaccharides in water, 
ChemPhotoChem, 4, 577-581 (2020)
DOI: 10.1002/cptc.202000022
 

2019

T. Ikeda, K. Ichikawa, H. Shigeto, T. Ishida, R. Hirota, H. Funabashi, A. Kuroda
Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish
Biosci., Biotech., Biochem., 83(12):2272-2275, 2019. doi: 10.1080/09168451.
 
H. Shigeto, T. Ono, T. Ikeda, R. Hirota, T. Ishida, A. Kuroda, and H. Funabashi
Insulin sensor cells for the analysis of insulin secretion responses in single living pancreatic β cells
Analyst, 144, 3765-3772, 2019 (8th May)

T. Ishida, N. Fujihara, T. Nishimura, H. Funabashi, R. Hirota, T. Ikeda, and A. Kuroda
Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy
Genes and Environment, 41, 14-25, 2019 (5th April)
 

2018

Motomura K, Sano K, Watanabe S, Kanbara A, Gamal Nasser AH, Ikeda T, Ishida T, Funabashi H, Kuroda A, Hirota R.
Synthetic Phosphorus Metabolic Pathway for Biosafety and Contamination Management of Cyanobacterial Cultivation.
ACS Synth Biol. 2018 Sep 11. doi: 10.1021/acssynbio.8b00199
 
A. Nakamura, N. Kawano, K. Motomura, A. Kuroda, K. Sekiguchi, M. Miyado, W. Kang, Y. Miyamoto, M. Hanai, M. Iwai, M. Yamada, T. Hamatani, T. Saito, H. Saito, M. Tanaka, K. Miyado, A. Umezawa,
Degradation of phosphate polymer polyP enhances lactic fermentation in mice,
Genes to Cells, 23(10):904-914, 2018.
 
Daiki Antoku, Shuhei Satake, Tomoya Mae, KoutaSugikawa, Hisakage Funabashi, Akio Kuroda, Atsushi Ikeda
Improvement of Photodynamic Activity of Lipid–Membrane-Incorporated Fullerene Derivative by Combination with aPhoto-Antenna Molecule
Chem. Eur.J., 24,7335 –7339 (April 6, 2018)
 

2017

Atsushi Ikeda, Shuhei Satake, Tomoya Mae, Masafumi Ueda, Kouta Sugikawa , Hajime Shigeto, Hisakage Funabashi, and Akio Kuroda
Photodynamic Activities of Porphyrin Derivative–Cyclodextrin Complexes by Photoirradiation
ACS Med. Chem. Lett., 8 (5), pp 555–559 (2017, April)
 
Ikeda A, Mae T, Ueda M, Sugikawa K, Shigeto H, Funabashi H, Kuroda A, Akiyama M.
Improved photodynamic activities of liposome-incorporated [60]fullerene derivatives bearing a polar group
Chem Commun (Camb)., 53(20):2966-2969 (2017 Mar 7)
 
Ryuichi Hirota, Kenji Abe, Zen-ichiro Katsuura, Reiji Noguchi, Shigeaki Moribe
Kei Motomura, Takenori Ishida, Maxym Alexandrov, Hisakage Funabashi, Takeshi Ikeda & Akio Kuroda
A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite
Scientific Reports, 7:44748 (2017 March)
 

2016

Kenji Abe, Akio Kuroda, Ryo Takeshita
Engineering of Escherichia coli to facilitate efficient utilization of isomaltose and panose in industrial glucose feedstock
Applied Microbiology and Biotechnology, doi:10.1007/s00253-016-8037-z (2016 Dec. 8).
 
Shigeto, Hajime; Nakatsuka, Keisuke; Ikeda, Takeshi; Hirota, Ryuichi; Kuroda, Akio; Funabashi, Hisakage
Continuous monitoring of specific mRNA expression responses with a FRET-based DNA nano-tweezer technique that does not require gene recombination
Analytical Chemistry, 2016, 88 (16), pp 7894–7898 (2016 Jul 26)
 
T. Nishimura, M. Alexandrov, T. Ishida, R. Hirota, T. Ikeda, K. Sekiguchi, A. Kuroda
Differential counting of asbestos using phase contrast and fluorescence microscopy
Annals of Occupational Hygiene, (2016) 60 (9): 1104-1115 (2016 Jul 22)
 
Kuroda, A, Alexandrov M., Nishimura T., Ishida T
Rapid on-site detection of airborne asbestos fibers and potentially hazardous nanomaterials using fluorescence microscopy-based biosensing
Biotechnol J. 11(6):757-767 (2016 May 24)
 
M. A.A. Abdelhamid, T. Ikeda, K. Motomura, T. Tanaka, T. Ishida, R. Hirota, A. Kuroda
Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag
Journal of Bioscience and Bioengineering,122 No. 5, 633-638, (May 2016)
 
T. Taniguchi, A. Hirowatari, T. Ikeda, M. Fukuyama, Y. Amemiya, A. Kuroda, S. Yokoyama
Detection of antibody-antigen reaction by silicon nitride slot-ring biosensors using protein G
Opt. Commun., 365, 16-23, (April 15, 2016)
 
T. Taniguchi, S. Yokoyama, Y. Amemiya, T. Ikeda, A. Kuroda, and S. Yokoyama,
Differential Si ring resonators for label-free biosensing
Japanese Journal of Applied Physics 55, 04EM04 (March, 11, 2016)
 
K.Motomura, T. Ikeda, S. Matsuyama, M. A. A. Abdelhamid, T. Tanaka, T. Ishida, R. Hirota, A. Kuroda,
The C-terminal zwitterionic sequence of CotB1 Is essential for biosilicification of the Bacillus cereus spore coat, J. Bacteriol., January 2016 Volume 198 Number 2: 276-282
 
A Saiki Y. Ishida, S. Segawa, R. Hirota, T. Nakamura, A. Kuroda,
A Lactobacillus mutant capable of accumulating long-chain polyphosphates that enhance intestinal barrier function, Bioscience, Biotechnology, and Biochemistry, 11:1-7, 2016 (Mar)
 

2015

K. Nakatsuka, H. Shigeto, A. Kuroda,H. Funabashi,
A split G-quadruplex-based DNA nano-tweezers structure as a signal-transducing molecule for the homogeneous detection of specific nucleic acids, Biosens. Bioelectron., 74, 222–226, 2015
 
A. Ikeda, S. Hino, T. Mae, K. Sugikawa,M. Tsukamoto, K. Ya-suhara,H. Shigeto, H. Funabashi, A. Kuroda and M. Akiyama,
Porphyrin-uptake in liposomes and living cells using an exchange method with cyclodextrin, RSC Adv., 5:105279–105287, 2015
 
K. Sekine, H. Yamamoto, S. Kono, T. Ikeda, A. Kuroda, T. Tanii
Surface modification of cell scaffold in aqueous solution using TiO2 photocatalysis and linker protein L2 for patterning primary neurons
e-Jounal of Surface Science and Nanotechnology, 13:213-218, 2015
 
K.-S. Ju, J. Gao, J. R. Doroghazi, K.-K. Wang, C. Thibodeaux, S. Li, E. Metzger, J. Fudala, J. Su, J. Zhang, J. P. Cioni, J. Lee, B. S. Evans, R. Hirota, D. P. Labeda, W. A. van der Donk, W. W. Metcalf
Discovery of phosphonic acid natural products by mining the genomes of 10,000 Actinomycetes, Proc. Nat. Acad. Sci. USA., 112(39), 12175-12180 (July 31, 2015)
 
H. Shigeto, T. Ikeda, A. Kuroda, H. Funabashi,
A BRET-Based Homogeneous Insulin Assay Using Interacting Domains in the Primary Binding Site of the Insulin Receptor
Analytical Chemistry, 87(5):2764-2770, 2015
 
A. Kuroda and R. Hirota
Environmental Biotechnology for Efficient Utilization of Industrial Phosphite Waste
Global Environmental Research, 19, 77-82, 2015
 
H. Funabashia, H. Shigeto, K. Nakatsuka, and A. Kuroda
A FRET-based DNA nano-tweezers technique for the imaging analysis of specific mRNA
Analyst, 140(4):999-1003, 2015
 
M. Alexandrov, E. Ichida, T, Nishimura, K. Aoki, T. Ishida, R. Hirota, T, Ikeda, T. Kawasaki, A. Kuroda
Development of an automated asbestos counting software based on fluorescence microscopy
Environ Monit Assess (2015) 187:4166
 

2014

T. Ikeda, T. Ueda, H. Tajima, K. Sekiguchi, and A. Kuroda
Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization
Anal. Methods (2014) 6, 6232-6235
 
K. Kanda, T. Ishida, R. Hirota, S. Ono, K. Motomura, T. Ikeda, K. Kitamura and A. Kuroda
Application of a phosphite dehydrogenase gene as a novel dominant selection
marker for yeasts
J. Biotechnol. 182-183:68-73 (2014)
 
M.A.A. Abdelhamid, K. Motomura, T. Ikeda, T. Ishida, R. Hirota, and A. Kuroda
Affinity purification of recombinant proteins using a novel silica-binding
peptide as a fusion tag
Appl. Microbiol. Biotechnol. 98(12):5677-5684 (2014)
 
K. Motomura, R. Hirota, M. Okada, T. Ikeda, T. Ishida, and A. Kuroda,
A new subfamily of polyphosphate kinase 2 (class III PPK2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation, Appl. Environ. Microbiol. 80(8), 2602-2608 (2014)
 

2013

T. Ishida, M. Alexandrov, T. Nishimura, K. Minakawa, R. Hirota, K. Sekiguchi, N. Kohyama, and A. Kuroda.
Molecular engineering of a fluorescent bioprobe for sensitive and selective detection of amphibole asbestos
PLos ONE, 8(9):e76231 (2013)
 
R. Hirota, K. Motomura, S. Nakai, T. Handa, T. Ikeda, A. Kuroda.
Stable polyphosphate accumulation by a pseudorevertant of an Escherichia coli phoU mutant
Biotechnol. Lett. 35(5):695-701 (2013)
 

2012

X. Ye, K. Honda, T. Sakai, K. Okano, T. Omasa, R. Hirota, A. Kuroda, H. Ohtake
Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway
Microbial Cell Factories 11, 120 (2012)
 
R. Hirota, S. Yamane, T. Fujibuchi, K. Motomura, T. Ishida, T. Ikeda, A. Kuroda
Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506
J. Biosci. Bioeng., 113, 445-450 (2012) doi:10.1016/j.jbiosc.2011.11.027
 
E. Restiawaty, K. Honda, K. Okano, R. Hirota, T. Omasa, A. Kuroda, H, Ohtake
Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis glycerol kinase and its application to repetitive batchwise reactions
J. Biosci. Bioeng., 113, 521-525 (2012) doi:10.1016/j.jbiosc.2011.11.016
 
T. Ishida, M. Alexandrov, T. Nishimura, K. Minakawa, R. Hirota, K. Sekiguchi, N. Kohyama, and A. Kuroda.
Evaluation of sensitivity of fluorescence-based asbestos detection by correlative microscopy.
J. Fluoresc., 22, 357-363 (2012) doi:10.1007/s10895-011-0967-3
 

2011

Y. Murakami, Y. Zhang, T. Takeuchi, T. Noda, K. Noda, A. Kuroda
Application of an enteric coat increases the resistance to gastric juices for
swallowable biosensors
Sensors and Actuators B, 160, 379-383 (2011)
 
E. Restiawaty, Y. Iwasa, S. Maya, K. Honda, T. Omasa, R. Hirota, A. Kuroda, H. Ohtake
Feasibility of thermophilic ATP regeneration system using Thermus thermophilus polyphosphate kinase.
Process Biochem., 46:1747-1752 (2011).doi:10.1016/j.procbio.2011.05.021
 
T. Ikeda, A. Kuroda
Why does the silica-binding protein "Si-tag" strongly bind to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces
Colloids Surf. B: Biointerfaces, 86(2), 359-363 (2011) doi:10.1016/j.colsurfb.2011.04.020
 
K. Motomura, R. Hirota, N. Ohnaka, M. Okada, T. Ikeda, T. Morohoshi, H. Ohtake, A. Kuroda
Overproduction of YjbB reduces the level of polyphosphate in Escherichia coli: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation
FEMS Microbiol. Lett., 320, 25-32 (2011) doi:10.1111/j.1574-6968.2011.02285.x
 
T. Ikeda, K. Motomura, Y. Agou, T. Ishida, R. Hirota, A. Kuroda
The silica-binding Si-tag functions as an affinity tag even under denaturing conditions
Protein Expr. Purif. 77, 173-177 (2011) doi:10.1016/j.pep.2011.01.012
 
M. Fukuyama, M. Nishida, Y. Abe, Y. Amemiya, T. Ikeda, A. Kuroda, S. Yokoyama
Detection of antigen-antibody reaction using Si ring optical resonators functionalized with an immobilized antibody-binding protein
Jpn. J. Appl. Phys., Appl. Phys., 50, 04DL07 (2011) doi:10.1143/JJAP.50.04DL07
 
M. Fukuyama, Y. Amemiya, Y. Abe, Y. Onishi, A. Hirowatari, K. Terao, T. Ikeda, A. Kuroda, S. Yokoyama
Sensitivity improvement of biosensors using Si ring optical resonators
Jpn. J. Appl. Phys., 50, 04DL11 (2011) doi:10.1143/JJAP.50.04DL11
 

2010

K. Honda, S. Maya, T. Omasa, R. Hirota, A. Kuroda, H. Ohtake
Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes
J. Biotechnol. 148, 204-207 (2010) doi:10.1016/j.jbiotec.2010.06.008
 
M. Fukuyama, S. Yamatogi, H. Ding, M. Nishida, C. Kawamoto, Y. Amemiya, T. Ikeda, T. Noda, S. Kawamoto, K. Ono, A. Kuroda, S. Yokoyama
Selective detection of antigen-antibody reaction using Si ring optical resonators
Jpn. J. Appl. Phys. 49, 04DL09 (2010) doi:10.1143/JJAP.49.04DL09
 
T. Ikeda, K. Ninomiya, R. Hirota, A. Kuroda
Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner
Protein Expr. Purif. 71, 91-95 (2010) doi:10.1016/j.pep.2009.12.009
 
R. Hirota, A. Kuroda, J. Kato, H. Ohtake
Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses
J. Biosci. Bioeng. 109, 423-432 (2010) Review doi:10.1016/j.jbiosc.2009.10.018
 
H. Kashihara, B. Mun Kang, T. Omasa, K. Honda, Y. Sameshima, A. Kuroda, H. Ohtake
Electron microscopic analysis of heat-induced leakage of polyphosphate from a phoU mutant of Escherichia coli
Biosci. Biotechnol. Biochem. 74, 865-868 (2010) doi:10.1271/bbb.90820
 
T. Ishida, M. Alexandrov, T. Nishimura, K. Minakawa, R. Hirota, K. Sekiguchi, N. Kohyama, A. Kuroda
Selective detection of airborne asbestos fibers using protein-based fluorescent probes
Environ. Sci. Technol. 44, 755-759 (2010) doi:10.1021/es902395h
 
R. Hirota, Y. Hata, T. Ikeda, T. Ishida, A. Kuroda
The silicon layer supports acid resistance of Bacillus cereus spore
J. Bacteriol. 192, 111-116 (2010) doi:10.1128/JB.00954-09
 
K. Noda, H. Goto, Y. Murakami, A.B.F. Ahmed, A. Kuroda
Endotoxin assay by bioluminescence using mutant firefly luciferase
Anal. Biochem. 397, 152-155 (2010) doi:10.1016/j.ab.2009.10.025
2009

T. Satoh, K. Tsuruta, Y. Shinoda, R. Hirota, K. Noda, A. Kuroda, Y. Murakami
Reciprocating-flow ATP amplification system for increasing the number of amplification cycles
Anal. Biochem. 395, 161-165 (2009) doi:10.1016/j.ab.2009.08.025
 
S. Mahboob, K. Makihara, A. Ohta, S. Higashi, Y. Hata, A. Kuroda, S. Miyazaki
Surface Potential Changes Induced by Physisorption of Si-tagged Protein A on HF-last Si(100) and Thermally Grown SiO2 surfaces
ECS Transactions 10/2009; 19(22). DOI:10.1149/1.3245167
 
T. Satoh, Y. Shinoda, S. Tokonami, R. Hirota, K. Noda, A. Kuroda, Y. Murakami
Continuous-flow ATP amplification system on a chip
Sens. Actuators B 142, 118-122 (2009) doi:10.1016/j.snb.2009.08.020
 
M. Iinuma, Y. Ushio, A. Kuroda, Y. Kadoya,
High-sensitivity detection of ATP using bioluminescence at an optical fiber end
Electron. Commun. Jpn., 92, 53-59 (2009) doi:10.1002/ecj.10054
 
M. Urata, R. Iwata, K. Noda, Y. Murakami, A. Kuroda
Detection of living Salmonella cells using bioluminescence
Biotechnol. Lett. 31, 737-741 (2009) doi:10.1007/s10529-009-9924-5
 
T. Kudo, T. Kasama, T. Ikeda, Y. Hata, S. Tokonami, S. Yokoyama, T. Kikkawa, H. Sunami, T. Ishikawa, M. Suzuki, K. Okuyama, T. Tabei, K. Ohkura, Y. Kayaba, Y. Tanushi, Y. Amemiya, Y. Cho, T. Monzen, Y. Murakami, A. Kuroda, A. Nakajima
Fabrication of Si nanowire field-effect transistor for highly sensitive, label-free biosensing
Jpn. J. Appl. Phys. 48, 06FJ04 (2009) doi:10.1143/JJAP.48.06FJ04
 
S. Yamatogi, Y. Amemiya, T. Ikeda, A. Kuroda, S. Yokoyama
Si ring optical resonators for integrated on-chip biosensing
Jpn. J. Appl. Phys. 48, 04C188 (2009) doi:10.1143/JJAP.48.04C188
 
T. Ikeda, Y. Hata, K. Ninomiya, Y. Ikura, K. Takeguchi, S. Aoyagi, R. Hirota, A. Kuroda
Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A
Anal. Biochem. 385, 131-137 (2009) doi:10.1016/j.ab.2008.11.001
 

2008

J. Kato, H.E. Kim, N. Takiguchi, A. Kuroda, H. Ohtake
Pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem
J Biosci. Bioeng. 106, 1-7 (2008) Review doi:10.1263/jbb.106.1
 
T. Satoh, Y. Shinoda, M. Alexandrov, A. Kuroda, Y. Murakami
Continuous-flow ATP amplification system for increasing the sensitivity of quantitative bioluminescence assay
Anal. Biochem. 379, 116-120 (2008) doi:10.1016/j.ab.2008.04.045
 
K. Noda, T. Matsuno, H. Fujii, T. Kogure, M. Urata, Y. Asami, A. Kuroda
Single bacterial cell detection using a mutant luciferase
Biotechnol. Lett. 30, 1051-1054 (2008) doi:10.1007/s10529-008-9643-3
 
K. Noda, T. Kogure, S. Irisa, Y. Murakami, M. Sakata, A. Kuroda
Enhanced dibenzothiophene biodesulfurization in a microchannel reactor
Biotechnol. Lett. 30, 451-454 (2008) doi:10.1007/s10529-007-9557-5
 
A. Kuroda, T. Nishimura, T. Ishida, R. Hirota, K. Nomura
Detection of chrysotile asbestos by using a chrysotile-binding protein
Biotechnol. Bioeng. 99, 285-289 (2008) doi:10.1002/bit.21588
 

2007

S. Iwamoto, K. Motomura, Y. Shinoda, M. Urata, J. Kato, N. Takiguchi, H. Ohtake, R. Hirota, A. Kuroda
Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate
Appl. Environ. Microbiol. 73, 5676-5678 (2007) doi:10.1128/AEM.00278-07
 
N. Takiguchi, M. Kishino, A. Kuroda, J. Kato, H. Ohtake
Effect of mineral elements on phosphorus release from heated sewage sludge
Bioresour. Technol. 98, 2533-2537 (2007) doi:10.1016/j.biortech.2006.09.015
 
A.B.F. Ahmed, K. Noguchi, Y. Asami, K.Nomura, H. Fujii, M. Sakata, A. Tokita, K. Noda, A. Kuroda
Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection
J. Biosci. Bioeng. 104, 55-61 (2007) doi:10.1263/jbb.104.55
 
H. Fujii, K. Noda, Y. Asami, A. Kuroda, M. Sakata, A. Tokida
Increase in bioluminescence intensity of firefly luciferase using genetic modification
Anal. Biochem. 366, 131-136 (2007) doi:10.1016/j.ab.2007.04.018
 
K. Taniguchi, K. Nomura, Y. Hata, T. Nishimura, Y. Asami, A. Kuroda
The Si-tag for immobilizing proteins on a silica surface
Biotechnol. Bioeng. 96, 1023-1029 (2007) doi:10.1002/bit.21208
 

2006

Y. Asami, T. Satoh, A. Kuroda
Polyphosphate-ATP amplification technology: principle and its application to specific bacteria detection
J. Environ. Biotechnol. 6, 105-108 (2006) Review
 
K. Motomura, N. Takiguchi, H. Ohtake, A. Kuroda
Polyamines affect polyphosphate accumulation in Escherichia coli
J. Environ. Biotechnol. 6, 41-46 (2006)
 
K Nomura, J. Kato, N. Takiguchi, H. Ohtake, A. Kuroda
Inorganic polyphosphate stimulates Lon-mediated proteolysis of nucleoid proteins in Escherichia coli
Cell. Mol. Biol. 52, 23-29 (2006)
 
H.-E. Kim, M. Shitashiro, A. Kuroda, N. Takiguchi, H. Ohtake, J. Kato
Identification and characterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene
J. Bacteriol. 188, 6700-6702 (2006) doi:10.1128/JB.00584-06
 
R. Hirota, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake, J. Kato
Transcriptional analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp. strain ENI-11
Biosci. Biotechnol. Biochem. 70, 1875-1881 (2006) doi:10.1271/bbb.60049
 

2005

I. Faizal, K. Dosen, C.S. Hong, A. Kuroda, N. Takiguchi, T. Ikeda, H. Ohtake, K. Takeda, H. Tsunekawa, J. Kato
Isolation and characterization of solvent-tolerant Pseudomonas putida strain T-57, and its application to biotransformation of toluene to cresol in a two-phase (organic-aqueous) system
J. Ind. Microbiol. Biotechnol. 32, 542-547 (2005) doi:10.1007/s10295-005-0253-y
 
K.-S. Na, K. Nagayasu, A. Kuroda, N. Takiguchi, T. Ikeda, H. Ohtake, J. Kato
Development of a genetic transformation system for benzene-tolerant Rhodococcus opacus strains
J. Biosci. Bioeng. 99, 408-414 (2005) doi:10.1263/jbb.99.408
 
C.-S. Hong, A. Kuroda, N. Takiguchi, H. Ohtake, J. Kato
Expression of Pseudomonas aeruginosa aer-2, one of two aerotaxis transducer genes is controlled by RpoS
J. Bacteriol. 187, 1533-1535 (2005) doi:10.1128/JB.187.4.1533-1535.2005
 

2004

N. Takiguchi, M. Kishino, A. Kuroda, J. Kato, H. Ohtake
A laboratory-scale test of anaerobic digestion and methane production after phosphorus recovery from waste activated sludge
J. Biosci. Bioeng. 97, 365-368 (2004)
 
K. Nomura, J. Kato, N. Takiguchi, H. Ohtake, A. Kuroda
Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli
J. Biol. Chem. 279, 34406-34410 (2004)
 
T. Satoh, J. Kato, N. Takiguchi, H. Ohtake, A. Kuroda
ATP amplification for ultrasensitive bioluminescence assay: detection of a single bacterial cell
Biosci. Biotechnol. Biochem. 68, 1216-1220 (2004)
 
L. Yang, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake, J. Kato
Characterization of pEC01, a novel plasmid required for phosphate taxis in Enterobacter cloacase IFO3320
Microbes Environ. 19, 45-52 (2004)
 
C.S. Hong, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake, J. Kato
The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by he anaerobic regulator ANR in Pseudomonasa aeruginosa
J. Biosci. Bioeng. 97, 184-190 (2004)
 
C.S. Hong, M. Shitashiro, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake, J. Kato
Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa
FEMS Microbiol. Lett. 231, 247-252 (2004)
 

2003

N. Takiguchi, A. Kuroda, J. Kato, K. Nukanobu, H. Ohtake
Pilot plant tests on the novel process for phosphorus recovery from municipal wastewater
J. Chem. Eng., 36, 1143-1146 (2003)
 
S. Tanaka, S.-O. Lee, K. Hamaoka, J. Kato, N. Takiguchi, K. Nakamura, H. Ohtake, A. Kuroda
Strictly polyphosphate-dependent glucokinase in a polyphosphate-accumulating bacterium Microlunatus phosphovorus
J. Bacteriol., 185, 5654-5656 (2003)
 
T. Tajima, N. Takiguchi, J. Kato, T. Ikeda, A. Kuroda, H. Ohtake
Mutants of the Nematode Caenorhabditis elegans that are defective specifically in their attraction to cycloheximide
J. Biosci. Bioeng., 96, 149-153 (2003)
 
T. Morohoshi, T. Yamashita, J. Kato, T. Ikeda, N. Takiguchi, H. Ohtake, A. Kuroda
A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater
J. Biosci. Bioeng., 95, 637-640 (2003)
 
M Shitashiro, J. Kato, T. Fukumura, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake
Evaluation of bacterial aerotaxis for its potential use in detecting the toxicity of chemicals to microorganisms
J. Biotechnol., 101, 11-18 (2003)
 

2002

T. Morohoshi, T. Maruo, Y. Shirai, J. Kato, T. Ikeda, N. Takiguchi, H. Ohtake, A. Kuroda
Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp.
Appl. Environ. Microbiol., 68, 4107-4110 (2002)
 
H. Ohtake, A Kuroda, J. Kato, N. Takiguchi
Development of novel biological process for recovering phosphorus from wastewater
J. Chem. Ind. Eng. (China), 53 Suppl., 21-22 (2002)
 
R. Hirota, J. Kato, H. Morita, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake
Isolation and characterization of cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase / oxygenase large and small subunits in Nitrosomonas sp. strain ENI-11
Biosci. Biotechnol. Biochem., 66, 632-635 (2002)
 
T. Ikeda, Y. Inoue, A. Suehiro, H. Ikeshoji, T. Ishida, N. Takiguchi, A. Kuroda, J. Kato, H. Ohtake
The effects of cyclodextrins on autoinducer activities of quorum sensing in Pseudomonas aeruginosa
J. Inclusion Phenomena, 44, 381-382 (2002)
 
A. Kuroda, N. Takiguchi, T. Gotanda, K. Nomura, J. Kato, T. Ikeda, H. Ohtake
A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling
Biotechnol. Bioeng., 78, 333-338 (2002)
 
S.-O. Lee, J. Kato, K. Nakashima, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake
Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28
Biosci. Biotechnol. Biochem., 66, 1366-1369 (2002)
 
N. Takiguchi, T. Tajima, K. Asayama, J. Kato, T. Ikeda, A. Kuroda, H. Ohtake
Behavioral responses of the ciliated protozoan Paramecium caudatum toward 2, 4-dichlorophenoxyacetic acid and its analogues
J. Biosci. Bioeng., 93, 416-420 (2002)
 

2001

A. Kuroda, K. Nomura, R. Ohtomo, J. Kato, T. Ikeda, N. Takiguchi, H. Ohtake, A. Kornberg
Role of inorganic polyphosphate promoting ribosomal protein degradation by the Lon protease in E. coli
Science, 293, 705-708 (2001)
 
S. Tanaka, A. Kuroda, J. Kato, T. Ikeda, N. Takiguchi, H. Ohtake
A sensitive method for detecting AMP by utilizing polyphosphate-dependent ATP regeneration and bioluminescence reactions
Biochem. Eng. J., 9, 193-197 (2001)
 
J. Kato, C. Nagata, L. Yang, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake
Isolation and characterization of the Enterobactor cloacae cheR mutant defective in phosphate taxis
Biosci. Biotechnol. Biochem., 65, 456-458 (2001)
 
T. Ikeda, K. Kajiyama, T. Kita, N. Takiguchi, A. Kuroda, J. Kato, H. Ohtake
The synthesis of optically pure enantiomers of N-acyl-homoserine lactone autoinducers and their analogues
Chem. Lett., 314-315 (2001)
 
T. Tajima, N. Watanabe, Y. Kogawa, N. Takiguchi, J. Kato, T. Ikeda, A. Kuroda, H. Ohtake
Chemotaxis of the nematode Caenorhabditis elegans toward cycloheximide and quinine hydrochloride
J. Biosci. Bioeng., 91, 322-324 (2001)

2000

A. Kuroda, H. Kunimoto, T. Morohoshi, T. Ikeda, J. Kato, N. Takiguchi, A. Miya, H. Ohtake
Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS
J. Biosci. Bioeng., 90, 688-690 (2000)
 
A. Kuroda, H. Ohtake
Molecular analysis of polyphosphate accumulation in bacteria
Biochemistry (Moscow), 65, 362-367 (2000)
 
R. Hirota, A. Yamagata, J. Kato, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake
Physical map location f the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11
J. Bacteriol., 182, 825-828 (2000)
 
S.-O. Lee, J. Kato, N. Takiguchi, A. Kuroda, T. Ikeda, A. Mitsutani, H. Ohtake
Involvement of an extracellular protease in the algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28
Appl. Environ. Microbiol., 66, 4334-4339 (2000)
 
A. Yamagata, R. Hirota, J. Kato, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake
Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11
Biosci. Biotechnol. Biochem., 64, 1754-1757 (2000)
 
H. Wu, J. Kato, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake
Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa
J. Bacteriol., 182, 3400-3404 (2000)
 

1999

A. Kuroda, S. Tanaka, T. Ikeda, J. Kato, N. Takiguchi, H. Ohtake, Inorganic polyphosphate kinase is required to stimulate protein degradation and for adaptation to amino acid starvation in Escherichia coli, Proc. Natl. Acad. Sci. USA, 96, 14264-14269 (1999).
 
A. Yamagata, J. Kato, R. Hirota, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake, Isolation and characterization of two cryptic plasmids in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11, J. Bacteriol., 181, 3375-3381 (1999).
 
H. Wu, H. Kosaka, J. Kato, A. Kuroda, T. Ikeda, N. Takigichi, H. Ohtake, Cloning and characterization of Pseudomonas putida genes encoding the phosphate-specific transport system, J. Biosci. Bioeng., 87, 273-279 (1999).
 
J. Kato, T. Nakamura, A. Kuroda, H. Ohtake, Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa, Biosci. Biotechnol. Biochem., 63, 155-161 (1999).
 
H. Ohtake, A. Kuroda, J. Kato, and T. Ikeda, Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater, Progress in Molecular and Subcellar Biology, 23, 299-311, (1999).
 

1998

H. Ohtake, J. Kato, A. Kuroda, H. Wu, T. Ikeda, Regulation of bacterial phosphate taxis and polyphosphate accumulation in response to phosphate starvation stress, J. Bioscience, 23, 491-499 (1998).
 
K. Imazu, S. Tanaka, A. Kuroda, Y. Anbe, J. Kato, H. Ohtake, Enhanced utilization of phosphonate and phosphite by Klebsiella aerogenes, Appl. Environ. Microbiol., 64, 3754-3758 (1998).
 
J. Kato, Y. Amie, Y. Murata, A. Kuroda, A. Mitsutani, H. Ohtake, Development of a genetic transformation system for an alga-lysing bacterium. Appl. Environ. Microbiol., 64, 2061-2064 (1998).
 

1997

A. Kuroda, A. Kornberg, Polyphosphate kinase as a nucleotide diphosphate kinase in Escherichia coli and Pseudomonas aeruginosa, Proc. Natl. Acad. Sci. USA, 94, 439-442 (1997).
 
A. Kuroda, H. Murphy, M. Cashel, A. Kornberg, Guanosine tetra- and pentaphosphate promote accumulation of inorganic polyphosphate in Escherichia coli, J. Biol. Chem., 272, 21240-21243 (1997).
 
K. Kusaka, K. Shibata, A. Kuroda, J. Kato, H. Ohtake, Isolation and characterization of Enterobacter cloacae mutants which are defective in chemotaxis toward inorganic phosphate, J. Bacteriol., 179, 6192-6195 (1997).
 
K. Taguchi, H. Fukutomi, A. Kuroda, J. Kato, H. Ohtake, Genetic identification of chemotactic transducers for amino acids in Pseudomonas aeruginosa, Microbiology, 143, 3223-3229 (1997).
 

1996

H. Ohtake, H. Wu, K. Imazu, Y. Anbe, J. Kato, A. Kuroda, Bacterial phosphonate degradation, phosphite oxidation and polyphosphate accumulation, Res. Conserv. Recycl., 18, 125-134 (1996).
 
K. Taguchi, H. Fukutomi, A. Kuroda, J. Kato, H. Ohtake, Molecular cloning of a CDP-diacylglycerol synthase gene of Pseudomonas aeruginosa, Gene, 172, 165-166 (1996).
 
T. Nikata, Y. Sakai, K. Shibata, J. Kato, A. Kuroda, H. Ohtake, Molecular analysis of the phosphate-specific transport(pst) operon of Pseudomonas aeruginosa, Mol. Gen. Genet., 250, 692-698 (1996).
 

1995

A. Kuroda, T. Kumano, K. Taguchi, T. Nikata, J. Kato, H. Ohtake, Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa, J. Bacteriol., 177, 7019-7025 (1995).
 
J. Sekiguchi, K. Akeo, H. Yamamoto, F. K. Khasanov, J. C. Alonso, A. Kuroda, Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis, J. Bacteriol., 177, 5582-5589 (1995).
 

1994

T. Tokunaga, M. H. Rashid, A. Kuroda, J. Sekiguchi, Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis, J. Bacteriol., 176, 5177-5180 (1994).
 

1993

A. Kuroda, Y. Asami, J. Sekiguchi, Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis, J. Bacteriol., 175, 6260-6268 (1993) .
 
Y. Oda, R. Nakayama, A. Kuroda, J. Sekiguchi, Molecular cloning, sequence analysis, and characterization of a new cell wall hydrolase, CwlL, of Bacillus licheniformis, Mol. Gen. Genet., 241, 380-388 (1993).
 
M. H. Rashid, A. Kuroda, J. Sekiguchi, Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility, FEMS Microbiol. Lett., 112,135-140 (1993).
 
A. Kuroda, J. Sekiguchi, High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation, J. Bacteriol., 175, 795-801 (1993).
 

1992

A. Kuroda, J. Sekiguchi, Characterization of the Bacillus subtilis CwbA protein which stimulates cell wall lytic amidases, FEMS Microbiol. Lett., 95, 109-114 (1992).
 
A. Kuroda, M. H. Rashid, J. Sekiguchi, Molecular cloning and sequencing of the upstream region of the major Bacillus subtilis autolysin gene: a modifier protein exhibiting sequence homology to the major autolysin and the spoIID product, J. Gen. Microbiol., 138, 1067-1076 (1992).
 
A. Kuroda, Y. Sugimoto, T. Funahashi, J. Sekiguchi, Genetic structure, isolation and characterization of a Bacillus licheniformis cell wall hydrolase, Mol. Gen. Genet., 234, 129-137 (1992).
 

1991

A. Kuroda, J. Sekiguchi, Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene, J. Bacteriol., 173, 7304-7312 (1991).
 
A. Kuroda, M. Imazeki, J. Sekiguchi, Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis, FEMS Microbiol. Lett., 81, 9-14 (1991).
 

1990

A. Kuroda, J. Sekiguchi, Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene, J. Gen. Microbiol., 136, 2209-2216 (1990).
 
J. Sekiguchi, H. Oshu, A. Kuroda, H. Moriyama, T. Akamatsu, Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation, J. Gen. Microbiol., 136, 1223-1230 (1990).
 

1989

T. Imanaka, A. Kuroda, H. Y. Wang, Protein engineering of penicillinase as affinity ligands for bioprocessing, J. Ferment. Bioeng., 67, 315-320 (1989).