[bioinfo]Nanoporeのベースコールクオリティを比較してみた。その二

MinKNOW (release 19.12.5)

General summary:
Mean read length: 6,006.3
Mean read quality: 12.3
Median read length: 3,532.0
Median read quality: 12.7
Number of reads: 172,664.0
Read length N50: 12,406.0
Total bases: 1,037,070,203.0
Number, percentage and megabases of reads above quality cutoffs
Q5: 172664 (100.0%) 1037.1Mb
Q7: 172664 (100.0%) 1037.1Mb
Q10: 143186 (82.9%) 900.4Mb
Q12: 106061 (61.4%) 700.0Mb
Q15: 7493 (4.3%) 26.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1: 18.5 (242)
2: 18.4 (291)
3: 18.4 (222)
4: 18.3 (418)
5: 18.3 (426)
Top 5 longest reads and their mean basecall quality score
1: 78972 (10.3)
2: 73960 (9.5)
3: 71546 (9.4)
4: 71123 (11.4)
5: 70049 (13.6)

Guppy GPU (v.3.5.1)

General summary:
Mean read length: 6,008.0
Mean read quality: 12.1
Median read length: 3,534.0
Median read quality: 12.6
Number of reads: 172,664.0
Read length N50: 12,398.0
Total bases: 1,037,358,216.0
Number, percentage and megabases of reads above quality cutoffs
Q5: 172149 (99.7%) 1037.3Mb
Q7: 167715 (97.1%) 1034.4Mb
Q10: 136102 (78.8%) 904.9Mb
Q12: 102572 (59.4%) 726.6Mb
Q15: 10643 (6.2%) 62.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1: 19.3 (442)
2: 18.9 (371)
3: 18.7 (820)
4: 18.2 (694)
5: 18.2 (1169)
Top 5 longest reads and their mean basecall quality score
1: 78864 (10.6)
2: 74095 (9.6)
3: 71656 (9.5)
4: 71161 (11.5)
5: 70031 (13.9)